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A kind of salt-tolerant anion exchange membrane chromatographic medium and its preparation method and application

A technology of anion-exchange membrane and chromatographic medium, which is applied in the field of salt-tolerant anion-exchange membrane chromatographic medium and its preparation, to achieve the effect of improving the hydrophilicity of the membrane surface, high selectivity, and reducing non-specific adsorption and membrane fouling

Active Publication Date: 2020-11-03
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is currently no relevant literature on the use of polydopamine to improve the hydrophilicity of organic / inorganic membranes and to couple salt-tolerant anion-exchange ligands for the separation of biopharmaceuticals

Method used

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  • A kind of salt-tolerant anion exchange membrane chromatographic medium and its preparation method and application
  • A kind of salt-tolerant anion exchange membrane chromatographic medium and its preparation method and application
  • A kind of salt-tolerant anion exchange membrane chromatographic medium and its preparation method and application

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Embodiment 1

[0052] In the present embodiment, the preparation method of the salt-tolerant anion-exchange membrane chromatographic medium is as follows (the preparation process can be visualized as figure 1 shown in the schematic diagram), specifically include the following steps:

[0053] Add 160 mg of dopamine molecules into 20 mL of 30 mM Tris-HCl buffer (pH 9.5), and at the same time place a piece of polypropylene membrane with a pore size of 2.0 microns ( figure 1 Middle mark 1) Immerse in the dopamine solution, then react in a shaker at 200 rpm at room temperature for 24 hours. After the reaction, the diaphragm was taken out and washed three times with deionized water. Then, the dopamine-coated membrane ( figure 1 Mark 2) Immerse in 2mg / mL agmatine and 0.5mg / mL polyallylamine ( figure 1 Mark 3) Tris-HCl buffer solution (pH = 9.0, 50 mL), react in a shaker at 200 rpm for 24 hours (30°C). After the reaction, rinse the membrane with deionized water. Finally, blow dry with nitrogen ...

Embodiment 2

[0059] In the present embodiment, the preparation method of the salt-tolerant anion-exchange membrane chromatographic medium is as follows (the preparation process can be visualized as figure 1 shown in the schematic diagram), specifically include the following steps:

[0060] Add 0.45mg of dopamine molecules into 45mL of 30mM sodium carbonate-sodium bicarbonate buffer (pH10.5), and at the same time put a piece of polyethersulfone membrane with a pore size of 1.0 micron ( figure 1 Middle mark 1) Immerse in the dopamine solution, then react in a shaker at 400 rpm at room temperature for 1 hour. After the reaction, the diaphragm was taken out and washed three times with deionized water. Then, the dopamine-coated membrane ( figure 1 Mark 2) Immerse in 2.0mg / mL tris(2-aminoethyl)amine and 40.0mg / mL polypropylene amine ( figure 1 The sodium carbonate-sodium bicarbonate buffer solution (pH=11.0, 40mL) marked in 3) was reacted in a shaker at 400 rpm for 1 hour (15°C). After the r...

Embodiment 3

[0066] In this embodiment, the preparation method of the salt-tolerant anion-exchange membrane chromatographic medium (its preparation process can be visualized as figure 1 The shown schematic diagram) specifically includes the following steps:

[0067] Add 90 mg of dopamine molecules into 50 mL of 30 mM Tris-HCl buffer (pH 7.2), and at the same time place a piece of polyvinylidene fluoride membrane with a pore size of 5.0 microns ( figure 1 Middle mark 1) Immerse in the dopamine solution, then react at room temperature for 36 hours with a rotating speed of 20 rpm in a shaker. After the reaction, the diaphragm was taken out and washed three times with deionized water. Then, the dopamine-coated membrane ( figure 1 Mark 2) immersed in 2.5mg / mL agmatine and 0.1mg / mL polyhexamethylene biguanide ( figure 1 The Tris-HCl buffer solution (pH=7.0, 50 mL) indicated in 3) was reacted in a shaker at a speed of 20 rpm for 48 hours (80° C.). After the reaction, rinse the membrane with d...

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Abstract

The invention relates to a high-salt tolerant anion exchange membrane chromatography medium, a preparation method and uses thereof, wherein the chromatography medium is a separation medium containinga polydopamine coating adhered in a microfiltration membrane, and a salt-tolerant anion exchange ligand is coupled to the polydopamine coating. According to the present invention, the salt-tolerant anion exchange membrane chromatography medium can be used for biopharmaceutical purification, wherein the service life can be substantially prolonged due to the high hydrophilicity and the high mechanical strength of the membrane chromatography medium during the high-flow drug purification; and the whole preparation process is efficient, simple, low cost, and environmentally friendly.

Description

technical field [0001] The invention belongs to the field of separation membranes, and in particular relates to a membrane separation medium and its preparation method and application, in particular to a salt-tolerant anion exchange membrane chromatographic medium and its preparation method and application. Background technique [0002] Membrane chromatography is a technology that combines membrane pore effect and chromatographic selective adsorption. Because the mass transfer in the membrane is in the form of convection, membrane chromatography has obvious advantages in operating speed. At the same time, the bed is thin and the processing capacity is large. Its purification operation time is much shorter than that of column chromatography (based on diffusion mass transfer). ). Therefore, membrane chromatography can produce biopharmaceuticals (proteins, vaccines, and peptides) more efficiently, while mild treatment conditions can better maintain protein activity and meet th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D67/00B01D69/02B01D69/12B01D15/36B01J20/286B01J41/12C07K1/18
Inventor 万印华樊金鑫罗建泉陈向荣
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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