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Test strip for detecting HIV (Human Immunodeficiency Virus) antibody in urea, detection cup and preparation method of test strip

A technology of test strips and antibodies, applied in the field of medicine, can solve the problems of high false positives in saliva, only qualitative detection, low sensitivity, etc., and achieve the effects of ensuring authenticity, reliability, convenient transportation, and wide cycle range

Pending Publication Date: 2017-12-26
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The only non-infectious / very infectious urine testing method approved by CFDA is ELISA, which takes a long time (3 hours) and can only be used for qualitative testing
The colloidal gold method, which can also be used for qualitative detection, is favored and considered as the first choice because of its simple operation and short time-consuming. However, due to the low urine antibody content and sensitivity problems, no similar products have been found in the existing market. In addition, , it has been reported that compared with blood, urine testing has a higher false positive problem in saliva

Method used

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  • Test strip for detecting HIV (Human Immunodeficiency Virus) antibody in urea, detection cup and preparation method of test strip
  • Test strip for detecting HIV (Human Immunodeficiency Virus) antibody in urea, detection cup and preparation method of test strip
  • Test strip for detecting HIV (Human Immunodeficiency Virus) antibody in urea, detection cup and preparation method of test strip

Examples

Experimental program
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Effect test

Embodiment 1

[0040] see figure 1 , a test strip for detecting HIV antibodies in urine according to the present embodiment, the test strip includes a base plate 3, and a sample pad 1 and a nitrocellulose membrane 4 arranged on the base plate 3, the sample pad 1 adopts The sample pad coating liquid is processed, and the two ends of the nitrocellulose membrane 4 are overlapped with the sample pad 1 and the absorbent paper 7 respectively, and the colloidal gold-labeled mouse anti-human IgG antibody is sprayed on the sample pad 1, and the colloidal gold Labeled gp160 antigen and colloidal gold labeled gp36 antigen, the nitrocellulose membrane 4 is provided with a first detection line 61 coated with HIV recombinant antigen gp41 and HIV recombinant antigen gp160, and a second detection line 62 coated with HIV recombinant antigen gp36 , and the control line 5 coated with goat anti-mouse antibody.

[0041] Wherein, the sample pad treatment liquid comprises Tris-HCl buffer solution, HPMC, PAA, rabb...

Embodiment 2

[0054] The structure and preparation method of the test strip for detecting HIV antibodies in urine of the present embodiment are all the same as in Example 1, except that the concentration of the colloidal gold-labeled gp160 antigen is 1.5 μg / ml; the colloidal gold-labeled mouse The concentration of the anti-human IgG antibody was 4.5 μg / ml; the concentration of the colloidal gold-labeled gp36 antigen was 4.5 μg / ml. The amount of the colloidal gold labeled raw material is 2ul / cm; the coating concentration of the HIV recombinant antigen gp41 is 0.5mg / ml, the coating concentration of the HIV recombinant antigen gp160 is 0.2mg / ml, and the HIV recombinant antigen The coating concentration of gp36 is 0.8mg / ml, and the dosage of the HIV recombinant antigen is 0.15ul / mm. The concentration of the goat anti-mouse antibody is 0.8mg / ml, and the dosage of the goat anti-mouse antibody is 0.2ul / mm.

[0055] The sample pad treatment solution in this embodiment includes Tris-HCl buffer solu...

Embodiment 3

[0058] The structure and preparation method of the test strip for detecting HIV antibodies in urine of this embodiment are all the same as in Example 1, except that the concentration of the colloidal gold-labeled gp160 antigen is 3.5 μg / ml; the colloidal gold-labeled mouse The concentration of the anti-human IgG antibody was 6.5 μg / ml; the concentration of the colloidal gold-labeled gp36 antigen was 6.5 μg / ml. The amount of the colloidal gold labeled raw material is 3.5ul / cm; the coating concentration of the HIV recombinant antigen gp41 is 2.0mg / ml, the coating concentration of the HIV recombinant antigen gp160 is 0.8mg / ml, the HIV recombinant The coating concentration of the antigen gp36 is 0.9mg / ml, and the dosage of the HIV recombinant antigen is 0.13ul / mm. The concentration of the goat anti-mouse antibody is 0.9mg / ml, and the dosage of the goat anti-mouse antibody is 0.15ul / mm.

[0059] The sample pad treatment solution of this embodiment includes Tris-HCl buffer solution...

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Abstract

The invention discloses a test strip for detecting an HIV (Human Immunodeficiency Virus) antibody in urea, a detection cup and a preparation method of the test strip. The test strip comprises a bottom plate, and a sample pad, a nitrocellulose membrane and water absorption paper which are arranged on the bottom plate, wherein two ends of the nitrocellulose membrane are in lap joint with the sample pad and the water absorption paper; a mouse anti-human IgG antibody marked by colloidal gold and gp160 antigen marked by colloidal gold are sprayed onto the end, close to the nitrocellulose membrane, on the sample pad; and a first detection line coated by HIV recombinant antigen gp41 and HIV recombinant antigen gp160 and a control line coated by a goat-anti-mouse antibody are arranged on the nitrocellulose membrane. Due to addition of the new antigen gp160, a detection omission phenomenon can be reduced by using the test strip disclosed by the invention when compared with that of a conventional gp41 detection method, and the test strip for detecting the HIV antibody in urea ensures reality and reliability of data through high-sensitivity urinalysis, and can be applied to large-scale preliminary screening of Aids.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically, the invention relates to a test strip for detecting HIV antibodies in urine, a detection cup and a preparation method thereof. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) is a virus that infects the cells of the human immune system. It destroys the immune cells of the human body and causes the immune system to lose its resistance, resulting in various diseases. According to statistics from the National Center for Disease Control and Prevention, since 2007, AIDS has become the infectious disease that has caused the highest death toll in China and continues to this day. It is worth noting that 32.1% of the infected people in China are still undetected. The only way to determine whether a person has HIV is to get an HIV test. Data show that the number of HIV tests in China increased rapidly between 2008 and 2015. According...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/54386G01N33/558G01N33/56988
Inventor 张秋平陈立李高辉
Owner GUANGZHOU WONDFO BIOTECH
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