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Test strip for detecting HIV antibody in urine, test line coating solution and preparation method thereof

A technology of test strips and detection lines, which is applied in the field of medicine, can solve the problems of not being able to adhere to nitrocellulose membranes in an energy state, and achieve the effects of improving the chromatographic form of the membrane surface, simple operation, and protecting personal privacy

Active Publication Date: 2018-02-23
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing coating buffer cannot disperse HIV recombinant antigen gp160 well, and cannot make it adhere to the nitrocellulose membrane in the lowest energy state

Method used

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  • Test strip for detecting HIV antibody in urine, test line coating solution and preparation method thereof
  • Test strip for detecting HIV antibody in urine, test line coating solution and preparation method thereof
  • Test strip for detecting HIV antibody in urine, test line coating solution and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] The coating buffer of the test strip used to detect HIV antibodies in urine of the present embodiment includes a pH of 7.4, 20mM Tris-HCl buffer, and S9 and urea; wherein, relative to every 100mL of Tris-HCl buffer, The added amount of the S9 is 0.1 g, and the added amount of the urea is 5 g.

[0051] The detection line coating solution of the test strip used to detect HIV antibodies in urine of this embodiment includes the coating buffer of this embodiment and HIV recombinant antigens, and the HIV recombinant antigens include antigens gp160 and Antigen gp41 at a concentration of 1.0 mg / ml.

[0052] The preparation method of the test line coating solution in this embodiment includes the following steps:

[0053] Prepare Tris-HCl buffer as the base solution, then add S9 and urea to obtain the coating buffer; then add HIV recombinant antigen to the coating buffer to obtain the detection line coating solution.

Embodiment 2

[0055] The coating buffer of the present embodiment comprises pH 7, 10mM Tris-HCl buffer, and S9 and urea; wherein, relative to the Tris-HCl buffer per 100mL, the amount of S9 added is 0.01g, the The amount of urea added was 2 g.

[0056] The detection line coating solution of the test strip used to detect HIV antibodies in urine of this embodiment includes the coating buffer of this embodiment and HIV recombinant antigens, and the HIV recombinant antigens include antigens gp160 and Antigen gp41 at a concentration of 0.5 mg / ml.

[0057] The preparation method of the detection line coating solution in this embodiment is the same as that in Embodiment 1.

Embodiment 3

[0059] The coating buffer solution of this embodiment includes a pH of 8, 50mM Tris-HCl buffer solution, and S9 and urea; wherein, relative to every 100mL of Tris-HCl buffer solution, the amount of S9 added is 1g, and the urea The added amount is 10g.

[0060] The detection line coating solution of the test strip used to detect HIV antibodies in urine of this embodiment includes the coating buffer of this embodiment and HIV recombinant antigens, and the HIV recombinant antigens include antigens gp160 and Antigen gp41 at a concentration of 2.0 mg / ml.

[0061] The preparation method of the detection line coating solution in this embodiment is the same as that in Embodiment 1.

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Abstract

The invention discloses a test strip for detecting a HIV antibody in urine, a test line coating solution and a preparation method thereof. The test line coating solution comprises a HIV recombinant antigen, 1-50mM of a Tris-HCl buffer solution, S9 and urea and has pH of 7-8. The test line coating solution of the test strip for detecting a HIV antibody in urine solves the problems of poor gp160 solubility, difficulty of coating on a nitrocellulose membrane, difficulty of labeling of colloidal gold and the occurrence of gold particle precipitation and dead gold defects through use of a surfactant as a cosolvent in the gp160 antigen dissolution reagent and use of S9 and urea in the coating buffer solution. The test strip for high sensitivity detection of a HIV antibody in urine guarantees data truth and reliability and can be used for large-scale AIDS screening.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically, the invention relates to a test strip for detecting HIV antibodies in urine, a coating buffer, a detection line coating and a preparation method thereof. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) is a virus that infects the cells of the human immune system. It destroys the immune cells of the human body and causes the immune system to lose its resistance, resulting in various diseases. According to statistics from the National Center for Disease Control and Prevention, since 2007, AIDS has become the infectious disease that has caused the highest death toll in China and continues to this day. It is worth noting that 32.1% of the infected people in China are still undetected. The only way to determine whether a person has HIV is to get an HIV test. Data show that the number of HIV tests in China increased rapidly betwee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
CPCG01N33/531G01N33/56983G01N2333/155
Inventor 张秋平陈立李高辉
Owner GUANGZHOU WONDFO BIOTECH
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