Micro-injection method of Bactrocera dorsalis eggs

Abstract

The invention discloses a micro-injection method of Bactrocera dorsalis eggs. The method comprises the following steps that black gauze used for placing the Bactrocera dorsalis eggs is fixed to the tube opening of a centrifuge tube, the centrifuge tube is reversed, then the Bactrocera dorsalis eggs are immersed into a sodium hypochlorite solution with the volume concentration of 1.5-2%, and the Bactrocera dorsalis eggs are sufficiently in contact with the sodium hypochlorite solution; the processed shelled eggs are washed with pure water so that the hypochlorite solution can be washed as clean as possible; the processed shelled eggs are picked up into a culture dish covered with wet filter paper; the Bactrocera dorsalis eggs are sorted in the horizontal direction in sequence under an anatomical lens; the Bactrocera dorsalis eggs are transferred to a slide, the backs of tweezers slightly slide across the slide and fix the slide, and the shelled eggs are dried; then water and saturated oil cover the surfaces of the shelled eggs; micro-injection is conducted. The micro-injection method solves the problems of dry difficulty and excessively large inner pressure of the Bactrocera dorsalis eggs in micro-injection, after injection is improved, the hatching rate of the eggs reaches 10-30%, and the infection rate reaches 15-50%.

Description

technical field [0001] The invention belongs to the field of pest control, and in particular relates to a microinjection method of Bactrocera dorsalis eggs Background technique [0002] Bactroceradorsalis (Bactroceradorsalis) is a dangerous pest that harms fruits and vegetables. It was first discovered in a citrus orchard in Taipei, Taiwan in 1911 in my country, and it was reported in mainland China in 1937. In view of the wide host range of the insect, rapid reproduction and serious harm to the fruit trade, many countries list it as a quarantine object. The use of environmentally friendly control methods to control pests is the current trend of pest control. The realization of large-scale breeding of Bactrocera dorsalis is conducive to the development of environmentally friendly control technologies, such as sterile insect technique (sterile insect technique, SIT) and the use of natural enemy insects (such as parasitic wasps) to control pests. [0003] In the large-scale ...

AI Technical Summary

Problems solved by technology

[0010] One of the purposes of the present invention provides a new microinjection method of Bactrocera dorsalis ovum, which solves the problems of Bacteralis dorsalis ovum being difficult to dry and excessive internal pressure in microinjection