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Somatic cell cloning method for mammals and culture fluid utilized in method

A technology of somatic cell cloning and cloning method, which is applied in the field of somatic cell cloning of animals, especially mammals, which can solve the problems of low cloning efficiency, reprogramming failure, incompleteness, etc., to improve the development rate and quality, improve the development rate, reduce Effect of number of apoptotic cells

Active Publication Date: 2017-12-12
天津力牧生物科技有限公司
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Problems solved by technology

[0003] At present, it is believed that the main reason for the low cloning efficiency is that the donor mammalian somatic cell nucleus has not been completely reprogrammed by the recipient ooplasm, that is, the reprogramming failure or incomplete
No changes in gene sequence are involved in this reprogramming process, mainly changes in epigenetic modifications

Method used

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  • Somatic cell cloning method for mammals and culture fluid utilized in method

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Embodiment Construction

[0031] The present invention can be further described by the following examples, however, the scope of the present invention is not limited to the following examples. Those skilled in the art can understand that various changes and modifications can be made in the present invention without departing from the spirit and scope of the present invention. The present invention provides general and / or specific descriptions of the materials and test methods used in the tests. While many of the materials and methods of manipulation which are employed for the purposes of the invention are well known in the art, the invention has been described here in as much detail as possible. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0032] In the mammalian somatic cell cloning method, the present invention provides a method for processing m...

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Abstract

The invention discloses a somatic cell cloning method for mammals such as cows, dogs or sheep and culture fluid utilized in the method. The method comprises following mammal somatic cell cloning embryo processing steps: (1) injecting to-be-transplanted mammal somatic cells into denucleated oocytes, carrying out electro-fusion, then selecting successfully fused mammal somatic cell cloning embryos in 1 hour after electro-fusion, and carrying out activation; and (2) after the activation, transferring the mammal somatic cell cloning embryos into G1.3 culture fluid, and carrying out culturing. The invention further relates to the culture fluid used in the method. The method disclosed by the invention presents the excellent technical effects described in the description.

Description

technical field [0001] The invention belongs to the technical field of somatic cell cloning of animals, especially mammals, and relates to a method for processing mammalian somatic cell cloned embryos, for example, to a method for processing bovine or canine somatic cell cloned embryos, in particular to a method based on somatic cell nucleus Processing method of transplanted mammalian, such as bovine or canine somatic cell cloned embryos. The method for processing mammalian, such as bovine or canine somatic cell cloned embryos of the present invention can overcome one or more defects in the prior art and present the advantages of one or more aspects of the present invention. Background technique [0002] Mammalian somatic cell cloning is a technology with great research and commercial value, which can be applied to therapeutic cloning, disease models, human organ transplantation, animal transgenic research, protection of endangered animal breeds, expansion of excellent lives...

Claims

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Application Information

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IPC IPC(8): C12N15/877C12N5/10
CPCC12N5/0609C12N15/877C12N2500/32C12N2500/40C12N2501/065C12N2501/998
Inventor 许晓椿王小武郭晶迟大明钟原王娜
Owner 天津力牧生物科技有限公司
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