A fermented crude extract of Streptococcus salivarius and its preparation method and application

Abstract

The invention provides a fermented crude extract of Streptococcus salivarius and its preparation method and application. The preparation method comprises the following steps: (1) inoculating Streptococcus salivarius CGMCC NO.13308 in M17 medium containing sucrose for fermentation , to obtain a fermented liquid, the gained fermented liquid is centrifuged to get the supernatant; (2) add ammonium sulfate to the supernatant obtained in step (1), and centrifugally collect the precipitate that separates out when the saturation of ammonium sulfate is 40~80%, and the The resulting precipitate was dissolved, dialyzed, and freeze-dried. The present invention adopts Streptococcus salivarius CGMCC NO.13308 strain for the first time to obtain fermented crude extract, the fermented crude extract of Streptococcus salivarius has inhibitory effect on Streptococcus mutans biofilm formation, discloses that Streptococcus salivarius CGMCC NO.13308 has fermentation The new use of generating inhibitors of Streptococcus mutans biofilm formation broadens the source of inhibitors of Streptococcus mutans biofilm formation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fermented crude extract of Streptococcus salivarius and its preparation method and application. Background technique [0002] Dental caries, commonly known as caries, tooth decay, is a bacterial disease, can be secondary to pulpitis and periodontitis, and even cause alveolar bone and jaw inflammation. If it is not treated in time, the lesion will continue to develop, forming cavities, and eventually the crown will be completely destroyed and disappeared. The final result of its development is tooth loss. Dental caries is characterized by high incidence and wide distribution. The disease is the main common disease of the oral cavity and one of the most common human diseases. The World Health Organization has listed it as one of the three major diseases of human beings, along with tumors and cardiovascular diseases. [0003] There are many factors that cause dental cari...

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Problems solved by technology

[0004] At present, most of the research reports on dental caries treatment focus on controlling or reducing the number of Streptococcus mutans in the oral cavity. However, a healthy oral environment is generally in a state where harmful bacteria and beneficial bacteria reach a balance. Excessive reduction of harmful bacteria will Bring the imbalance of oral flora, and then cause other adverse reactions of the human body

In addition, some clinically used bactericidal drugs against Streptococcus mutans, such as silver ammonium nitrate and other compounds, have strong side effects, and long-term use will stain teeth, so they are not suitable for anterior teeth treatment

However, reducing the production of Streptococcus mutans biofilm can also reduce the risk of dental caries without destroying the inherent balance of oral flora or causing side effects, but there are relatively few reports in this area, especially the research related to microorganisms is extremely limited, and it can be concluded that Applied microbial resources are quite scarce

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