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Preparation method of polar-region beneficial bacteria elements and application thereof

A technology of beneficial bacteria and bacterial liquid, which is applied in the field of preparation of polar beneficial bacteria, can solve the problem that the beneficial bacteria cannot meet the requirements of various breeding objects and breeding environments, so as to improve the survival rate and weight gain rate, and reduce the incidence of rate and improve the effect of aquaculture water quality

Inactive Publication Date: 2017-12-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] A single prebiotic cannot meet the requirements of a variety of breeding objects and breeding environments, and the preparation of compound polar prebiotics is an effective way to solve aquaculture problems

Method used

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  • Preparation method of polar-region beneficial bacteria elements and application thereof
  • Preparation method of polar-region beneficial bacteria elements and application thereof
  • Preparation method of polar-region beneficial bacteria elements and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A polar probiotic element for aquaculture, the preparation method of which comprises the following steps:

[0029] 1) Cultivation of Low Temperature Bacillus, Low Temperature Lactobacillus and Antimicrobial Peptide Yeast: Take Low Temperature Bacillus, Lactobacillus and Antimicrobial Peptide Yeast from the test tube slant culture medium and inoculate them into Erlenmeyer flasks containing liquid medium respectively , and then placed in a shaker, cultured at 20°C and 150r / min for 24h;

[0030] 2) Inoculate the low-temperature bacillus liquid, lactobacillus liquid and antimicrobial peptide yeast liquid in the logarithmic phase of step 1) into the fermentation medium at a ratio of 1:1:1 for fermentation. The fermentation temperature is 20°C. Fermentation time is 72h;

[0031] 3) Centrifuge the fermentation broth of the strains in the stable phase in step 2) at 10,000r / min for 20min to separate the bacteria and supernatant; add the obtained bacteria to the aqueous solution...

Embodiment 2

[0036] A polar probiotic element for aquaculture, the preparation method of which comprises the following steps:

[0037]1) Cultivation of Low Temperature Bacillus, Low Temperature Lactobacillus and Antimicrobial Peptide Yeast: Take Low Temperature Bacillus, Lactobacillus and Antimicrobial Peptide Yeast from the test tube slant culture medium and inoculate them into Erlenmeyer flasks containing liquid medium respectively , and then placed in a shaker, cultured at 20°C and 200r / min for 24h;

[0038] 2) Inoculate the low-temperature bacillus liquid, lactobacillus liquid and antimicrobial peptide yeast liquid in the logarithmic phase of step 1) into the fermentation medium at a ratio of 1:1:1 for fermentation. The fermentation temperature is 20°C. Fermentation time is 60h;

[0039] 3) Centrifuge the fermentation broth of the strains in the stable phase in step 2) at 10,000r / min for 20min to separate the bacteria and supernatant; add the obtained bacteria to the aqueous solution ...

Embodiment 3

[0044] A polar probiotic element for aquaculture, the preparation method of which comprises the following steps:

[0045] 1) Cultivation of Low Temperature Bacillus, Low Temperature Lactobacillus and Antimicrobial Peptide Yeast: Take Low Temperature Bacillus, Lactobacillus and Antimicrobial Peptide Yeast from the test tube slant culture medium and inoculate them into Erlenmeyer flasks containing liquid medium respectively , and then placed in a shaker, cultivated at 20°C, 150-250r / min for 24h;

[0046] 2) Inoculate the low-temperature bacillus liquid, lactobacillus liquid and antimicrobial peptide yeast liquid in the logarithmic phase of step 1) into the fermentation medium at a ratio of 1:1:1 for fermentation. The fermentation temperature is 20°C. Fermentation time is 48h;

[0047] 3) Centrifuge the fermentation broth of the strains in the stable phase in step 2) at 10,000r / min for 20min to separate the bacteria and supernatant; add the obtained bacteria to the aqueous solut...

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Abstract

The invention discloses a preparation method of polar-region beneficial bacteria elements and an application thereof. The preparation method includes: respectively subjecting low-temperature bacillus, low-temperature lactobacillus and antimicrobial peptides which are excellent in growth conditions to cultivation, fermentation and centrifugation; adding bacteria obtained from separation to a protective agent containing xylooligosaccharide, chitosan oligosaccharide, beta-glucosan, mycose, skimmed milk and vitamin C for evenly mixing before performing vacuum freeze drying to obtain the polar-region beneficial bacterial elements. The obtained polar-region beneficial bacterial elements can improve environment of aquaculture water, effective remove ammonia nitrogen and nitrite in the aquaculture water and inhibit growth of harmful pathogens in the aquaculture water, growth of fish is promoted, and survival rate of aquatic seedlings can be remarkably increased.

Description

technical field [0001] The invention belongs to the field of aquatic feed additives, and in particular relates to a preparation method and application of polar prebiotics. Background technique [0002] With the continuous development of the domestic economy, the demand for sustainable development of the aquaculture industry continues to increase. The high-density farming model and the abuse of antibiotics have seriously affected the development of the aquaculture industry. In the long run, it has led to an imbalance in the aquaculture ecological environment and frequent various diseases. occur. Furthermore, the long-term accumulation of antibiotics in aquatic animals also poses a potential threat to human health. Therefore, it is imperative to change the farming model and find alternatives to antibiotics. [0003] Polar Probiotics is a new type of product that can replace antibiotics. It is healthy and pollution-free. Long-term use can effectively change the microenvironme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/18C12R1/225
CPCC12N1/20A23K10/18C12N1/16
Inventor 陈宪刘旸李智杨明陈畅鸣
Owner FUZHOU UNIV
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