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Artificial feeding method for virus vector insect bemisia tabaci

A Bemisia tabaci, artificial technology, applied in the directions of botanical equipment and methods, biochemical equipment and methods, culture medium, etc., can solve the problems of prolonging the test time, slow development, hindering the test process, etc. The effect of ensuring vitality and shortening development time

Inactive Publication Date: 2017-12-01
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the three important factors involving host plants, viruses and whiteflies, the current development of this technology is slow. As long as there is a mistake in one link, the test time will be extended and the test process will be hindered.

Method used

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  • Artificial feeding method for virus vector insect bemisia tabaci
  • Artificial feeding method for virus vector insect bemisia tabaci

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Embodiment 1

[0034] A kind of artificial breeding method of virus vector insect Bemisia tabaci of the present invention, see figure 1 , including the steps of host plant cultivation, virus inoculation, whitefly inoculation, whitefly breeding, insect extraction, etc., specifically:

[0035] (1) Host plant cultivation:

[0036] 1.1. The host plants are commonly used vegetables such as tomato, pepper, cucumber, etc. In this example, tomato is used because tomato is the host of various plant viruses.

[0037] 1.2. The plants to be tested are kept in a clean insect-free cage. The insect-free cage is a frame cage made of aluminum alloy with a size of 60cm×40cm×80cm. Be to make with 100 order nylon nets, the front lower end is the door that 100 orders nylon nets are made (is the non-insect cage commonly used of raising Bemisia tabaci).

[0038] 1.3. Mixing peat, vermiculite, organic fertilizer and perlite in a mass ratio of 10:10:10:1 is used as a substrate for cultivating host plants.

[0039...

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Abstract

The invention discloses an artificial feeding method for virus vector insect bemisia tabaci. The method comprises the following steps that vegetables with main leaves are adopted as host plants; infectivity clone bacteria liquor is inoculated to the host plant; culture continues to be performed, and plants infected with diseases and non-toxic plants are screened out; B bemisia tabaci and Q bemisia tabaci are inoculated to the plants infected with diseases and the non-toxic platns to obtain the plants infected with diseases of B bemisia tabaci, the plants infected with diseases of Q bemisia tabaci, the non-toxic plants inoculated with the B bemisia tabaci and the non-toxic plants inoculated with the Q bemisia tabaci; independent feeding is performed, bemisia tabaci is bred; after feeding is performed for 6 generations, bemisia tabaci is taken out. According to the artificial feeding method, the insect developing time can be shortened, the egg laying amount is increased, the survival rate and stability are ensured, and the continuous and stable supply of bemisia tabaci is ensured.

Description

technical field [0001] The invention relates to the technical field of insect feeding, in particular to an artificial feeding method of the virus vector insect Bemisia tabaci. Background technique [0002] Bemisia tabaci has caused very serious losses to vegetables and food crops. There are mainly two types of Bemisia tabaci in my country: B and Q. Bemisia tabaci can transmit a variety of plant viruses, and the damage caused by the viruses transmitted by them is far higher than that caused by their own feeding. In order to control the virus transmission by whiteflies, the mechanism of virus transmission by whiteflies must first be studied. Therefore, it is necessary to establish a continuous supply of whiteflies that carry the virus and have a stable population, and have non-toxic tobacco that is raised under the same conditions. Whitefly served as a control. At the same time, in order to eliminate the influence of compound virus infection of field plants, plants must be i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01G31/00C12N15/82
CPCA01G31/00A01K67/033A01G24/00C12N15/8203
Inventor 史晓斌刘勇张德咏张友军张战泓
Owner HUNAN PLANT PROTECTION INST
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