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Method for preparing high-purity sophocarpidine from euchresta japonica

A matrine and high-purity technology, which is applied in the field of preparing high-purity matrine, can solve the problems of low yield, harsh conditions, and high yield, and achieve the effects of increasing yield, increasing rate, and simplifying the process flow

Inactive Publication Date: 2017-11-28
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Common extraction methods for matrine include solvent extraction, ion exchange, resin adsorption, supercritical extraction, membrane separation, and molecular imprinting separation. Each separation method has its advantages and disadvantages. Some methods have high yields, but the conditions are harsh; Some methods are simple, but the yield is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for preparing high-purity matrine from Shandougen, the specific operation is as follows:

[0015] Dry and pulverize the shandougen, put it in an ultrasonic extractor, add an appropriate amount of reducing agent sodium metabisulfite and 30% ethanol, the mass ratio of sodium metabisulfite to shandougen powder is 0.08:1, the ratio of ethanol to shandougen powder The mass ratio is 10:1. After immersion for 30 minutes, the ultrasonic power is 300W, the temperature is 80°C, the ultrasonic reduction is 30min, and the extraction is performed 4 times. The extracts are combined and the residue is discarded. The extract is concentrated. .The concentrated solution is allowed to stand overnight and then filtered to remove starch impurities and some tannins and proteins. After the filtrate was statically adsorbed by H103 macroporous resin, it was eluted with ethanol. The eluate was concentrated to dryness and dissolved in water. The aqueous solution was acidified with concentr...

Embodiment 2

[0017] A method for preparing high-purity matrine from Shandougen, the specific operation is as follows:

[0018] Dry and pulverize the shandougen, put it in an ultrasonic extractor, add an appropriate amount of reducing agent sodium sulfite and 60% ethanol, the mass ratio of sodium sulfite to shandougen powder is 0.09, and the mass ratio of ethanol to shandougen powder is 10 :1. After immersing for 60 minutes, ultrasonic power is 300W, temperature is 70℃, ultrasonic reduction is 60min, extraction is performed 4 times, the extracts are combined, and the residue is discarded. The extract is concentrated. .The concentrated solution is allowed to stand overnight and then filtered to remove starch impurities and some tannins and proteins. After the filtrate is statically adsorbed by SP825 macroporous resin, it is eluted with ethanol. The eluate is concentrated to dryness and dissolved in water. The aqueous solution is acidified with concentrated sulfuric acid to pH=3 to 4, and the a...

Embodiment 3

[0020] A method for preparing high-purity matrine from Shandougen, the specific operation is as follows:

[0021] Dry and crush the shandougen, put it in an ultrasonic extractor, add an appropriate amount of reducing agent sodium bisulfite and 70% ethanol, the mass ratio of sodium sulfite to shandougen powder is 0.1, the quality of ethanol and shandougen powder The ratio is 20:1, after immersing for 45 minutes, the ultrasonic power is 300W, the temperature is 60°C, the ultrasonic reduction is 45min, the extraction is 4 times, the extracts are combined, and the residue is discarded. The extract is concentrated. .The concentrated solution is allowed to stand overnight and then filtered to remove starch impurities and some tannins and proteins. After the filtrate is statically adsorbed by X-5 macroporous resin, it is eluted with ethanol. The eluate is concentrated to dryness and dissolved in water. The aqueous solution is acidified with concentrated sulfuric acid to pH=3~4, and the...

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PUM

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Abstract

The invention discloses a method for preparing high-purity sophocarpidine from euchresta japonica. An ultrasonic reduction method is adopted to extract sophocarpidine from euchresta japonica; a reducing agent, namely sodium metabisulfite, is added in the ultrasonic extraction process; oxymatrine, oxysophocarpine and sophocarpine can be converted into sophocarpidine, and thus the yield of the sophocarpidine is increased. The method comprises the following steps: leaving an ethanol extract to stand overnight, performing static adsorption and desorption with a macroporous resin, performing concentration and water dissolution on an ethanol eluant, adjusting the pH value to 2-7 with hydrochloric acid, filtering so as to remove precipitate, extracting filtrate with petroleum ether, performing alkalization with a sodium hydroxide solution till the pH value is 8-11, extracting with petroleum ether for multiple times till no alkaloid is detected, cooling and leaving the petroleum ether to stand, and separating out sophocarpidine crystal. By adopting the method, the yield of high-purity sophocarpidine prepared from euchresta japonica is increased, toxic organic solvents are not used, the process is simplified, good industrial practicability is achieved, and on-scale production can be met.

Description

Technical field [0001] The invention relates to a method for preparing matrine, in particular to a method for preparing high-purity matrine from the root of shandou. Background technique [0002] Shandougen is the dry root and rhizome of the leguminous shrub-like plant of Sophora japonicus, mainly produced in Guangxi, Guizhou, Yunnan and other places. Shandougen is used as medicine from its roots. Its taste is bitter, cold in nature and toxic. It has the effects of clearing heat, reducing swelling and throat, anti-tumor, anti-bacterial and anti-inflammatory. It is commonly used to treat primary liver cancer, various types of arrhythmias and various infectious diseases (Such as pharyngitis, hepatitis) and so on. The active ingredients of Shandougen are alkaloids, mainly matrine and oxymatrine, which have significant pharmacological activity. [0003] Common extraction methods for matrine include solvent extraction, ion exchange, resin adsorption, supercritical extraction, membrane...

Claims

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Application Information

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IPC IPC(8): C07D471/22
CPCC07D471/22
Inventor 王立升曹齐金蒋敏捷周中杰粱鹏云刘旭
Owner GUANGXI UNIV
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