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Method for extracting fustin and rhoifolin from rhus punjabensis var. sinica rehd

A technology of yellow lignin and anacardoside, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., to achieve the effects of improving purity, simplifying operation steps, and reducing production costs

Inactive Publication Date: 2017-11-24
长沙湘资生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently at home and abroad, mainly from the saponins of the leguminous plant saponins and pine cypresses to extract jaundice, but the research on extracting jasmine and rhododin from the seeds of the wild sumac tree has not been reported at home and abroad.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] Pulverize 100 kg of wild sumac seeds, add cellulase powder to a final concentration of 180 U / kg, stir evenly, then add 10% Bacillus subtilis liquid and ferment at 28°C for 3 days to obtain a fermented liquid. Use 0.04mol / L sodium hydroxide to adjust the pH value of the fermentation broth to 8.5, leave it at room temperature for 5 hours, add 450L of 75% ethanol aqueous solution, heat to 80°C for reflux extraction for 6 hours, and filter the extract once with a ceramic membrane to obtain about 400L of filtrate Ⅰ , and recovered ethanol under reduced pressure at 55°C, concentrated to a thick paste, and dried in a vacuum oven to a constant mass to obtain 6.75kg of crude extract. Dissolve the crude extract with 6.75 kg of 85% ethanol aqueous solution, put it on the XDA-8 macroporous resin column, and elute with 15% ethanol aqueous solution of three column volumes, and then elute with 70% ethanol aqueous solution of five column volumes to obtain 36 L wash After dehydration, t...

Embodiment 2

[0028] Pulverize 200 kg of wild sumac seeds, add cellulase powder to a final concentration of 200 U / kg, stir well, then add 10% Bacillus subtilis liquid and ferment at 28° C. for 60 h to obtain a fermented liquid. Adjust the pH value of the fermentation broth to 8.5 with 0.04mol / L sodium hydroxide, leave it at room temperature for 4 hours, add 800L of 75% ethanol aqueous solution, heat to 80°C for reflux extraction for 6 hours, and filter the extract once with a ceramic membrane to obtain 700L of filtrate I. Ethanol was recovered under reduced pressure at 55°C, concentrated to a thick paste, and dried in a vacuum oven to a constant mass to obtain 13.88 kg of a crude extract. Dissolve the crude extract with 13.88 kg of 80% ethanol aqueous solution, put it on the XDA‐8 macroporous resin column, and elute with 18% ethanol aqueous solution of three column volumes, and then elute with 75% ethanol aqueous solution of five column volumes to obtain 65 L wash After dehydration, the eth...

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Abstract

The invention provides a method for extracting and purifying fustin and rhoifolin from rhus punjabensis var. sinica rehd seeds. The method comprises the following steps: performing enzyme and microbial fermentation treatment, performing reflux extraction by adopting alkali alcohol water, filtering, concentrating till being dry, dissolving in alcohol water and then pouring onto a macroporous resin column, crystallizing under high temperature and re-crystallizing, thereby acquiring the pure products of fustin and rhoifolin. Through an enzyme method and alkali extraction, the extraction ratio of fustin and rhoifolin is greatly increased and the influence of the protein in the rhus punjabensis var. sinica rehd on the extraction is reduced. The combination of column chromatography and crystallization is adopted for separating, so that the purity of fustin and rhoifolin is greatly increased, the operation steps are simplified and the production cost of the fustin and rhoifolin is reduced.

Description

technical field [0001] The invention belongs to the technical field of natural products, and relates to a preparation method of jaundice and rhubaricin, and more specifically relates to a method for extracting and purifying jaundice and rhubaricin from the seeds of rhubarb. Background technique [0002] Wild sumac (Rhus punjabensis var.sinica Rehd.) is a small deciduous tree of the Rhus punjabensis genus Salina. Its roots, stems, leaves, bark, flowers and fruits are all used as medicine, and it does not need to be compatible with other traditional Chinese medicines. It is very effective when used alone Good, it has the effects of dispelling wind and dampness, astringent and detoxification, promoting body fluid and moistening lung, reducing fire and reducing phlegm. The galls formed on the young branches and leaves of this plant are commonly known as gallnuts, which can be used as important industrial raw materials. Flavonoids (3, 7, 3, 4-tetrahydroxyflavone) are flavonoids ...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40C07H17/07C07H1/08
CPCC07D311/30C07D311/40C07H1/08C07H17/07
Inventor 向华
Owner 长沙湘资生物科技有限公司
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