Kit and application of canine granulosa and Echinococcus multilocularis based on Pockit Micro fluorescent PCR platform

Abstract

The invention discloses a kit of dog echinococcus granulosus and echinococcus multilocularis based on POCKIT Micro fluorescent PCR platform, and application of the kit. The kit comprises fluorescent quantitative PCR reaction fluid, positive comparison product and negative comparison product; the fluorescent quantitative PCR reaction fluid comprises upstream general primers of echinococcus granulosus and echinococcus multilocularis, downstream general primers of echinococcus granulosus and echinococcus multilocularis, general probes of echinococcus granulosus and echinococcus multilocularis, Taq enzyme; premixing buffer and deionized water. The nucleotide sequence of the upstream general primers of echinococcus granulosus and echinococcus multilocularis and the nucleotide sequence of the downstream general primers of echinococcus granulosus and echinococcus multilocularis are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The kit design is specific to the general primers and the probes of two hydatids; the result can be determined by detection once, thus the time is greatly saved, and detection cost is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit and application of canine granule and Echinococcus multilocularis based on the POCKIT Micro fluorescent PCR platform. Background technique [0002] Echinococcus granulosus and multilocular echinococcosis is a serious zoonotic parasitic disease, which is common in Sichuan, Qinghai, Gansu, Ningxia, Xinjiang and other regions in my country. Among them, yak and sheep are Echinococcus granulosa As the intermediate host of tapeworms, prairie rodents are the intermediate hosts of Echinococcus multilocularis, and both dogs and foxes are the final hosts of these two kinds of echinococcosis. On the one hand, cattle and sheep infected with Echinococcus granulosus seriously affect their growth performance, and on the other hand, the viscera of cattle and sheep infected with Echinococcus granulosus are swallowed by dogs and foxes without treatment, which is the key to causing the...

AI Technical Summary

Problems solved by technology

[0003] At present, the grass-roots animal quarantine department mainly uses ELISA method for the detection of echinococcosis in dogs and foxes, which has poor specificity and low sensitivity, and it is easy to cause cross-reaction with other parasites (such as Taenia polycephala, etc.), so it cannot be accurately determined

The specialized laboratory testing is mainly the PCR method, which requires a special fluorescent PCR instrument, which is expensive

In addition, these current PCR methods are all primers and probes designed separately for Echinococcus granulosus or Echinococcus granulosus. For one sample, it needs to be tested twice, which is time-consuming, increases costs, and may even be affected due to technical problems. Weak and inoperable

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