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A HPLC method for the determination of L-carvone and D-carvone in spearmint oil

A technology of L-carvone and D-carvone, which is applied in the field of food analysis, can solve the problems of easy loss of column efficiency, unsuitable analysis of spearmint oil, and high price of chiral chromatographic columns, and achieve wide linear range, The effect of high sensitivity

Active Publication Date: 2019-04-12
淮安琼林科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

However, those skilled in the art know that the chiral chromatographic column is expensive, and the column efficiency is easy to lose, so it is not suitable for analyzing the spearmint oil with complex components.

Method used

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  • A HPLC method for the determination of L-carvone and D-carvone in spearmint oil
  • A HPLC method for the determination of L-carvone and D-carvone in spearmint oil
  • A HPLC method for the determination of L-carvone and D-carvone in spearmint oil

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Embodiment Construction

[0031] The substantive content of the present invention will be described in detail below in conjunction with the embodiments, but the protection scope of the present invention is not limited thereto.

[0032] 1. Experimental materials

[0033] 1 / 10,000 electronic balance (Sartorius);

[0034] LC-20A high performance liquid chromatography (Shimadzu);

[0035] Innoval ASB C18 acid-resistant chromatographic column (Bona Ajel);

[0036] L-carvone and D-carvone standard products are self-made (purity 98%), and the chemical structural formula is as figure 1 shown;

[0037] Spearmint oil was purchased from Nanjing Wensenbao International Trade Co., Ltd. (carvone content ≥ 65%);

[0038] Acetonitrile is chromatographically pure, water is deionized water, and other reagents are analytically pure, and the mobile phase is filtered through a 0.22 μm filter membrane before use.

[0039] 2. Experimental method and experimental results

[0040] 1. Solution preparation

[0041] Diluti...

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Abstract

The invention discloses an HPLC method for determining L(-)-carvone and D(+)-carvone in spearmint oil, wherein the stationary phase is an octadecyl-bonded silica gel, the mobile phase is an acetonitrile aqueous solution, and the acetonitrile aqueous solution contains effective concentrations of morpholine-based acidic ionic liquids specifically such as 4-methylmorpholine hydrosulfate, morphine hydrosulfate, and 4-(3-sulfopropyl)morphine hydrosulfate. According to the present invention, with the HPLC method, L(-)-carvone and D(+)-carvone can be effectively separated; and with the application of the HPLC method in the determination of L(-)-carvone and D(+)-carvone in spearmint oil, L(-)-carvone and D(+)-carvone can achieve baseline separation, the interference caused by other impurity peaks cannot be generated, the sensitivity is high, the linear range is wide, and the HPLC method can be used for the quality control of spearmint oil.

Description

technical field [0001] The invention belongs to the field of food analysis and relates to the separation and detection of chiral components in food, in particular to an HPLC method for determining L-carvone and D-carvone in spearmint oil. Background technique [0002] Chiral fragrances are an important class of compounds, and different enantiomers of fragrances may exhibit different aroma characteristics, aroma intensity, and biological activity. Carvone contains a chiral carbon atom, and is a chiral spice for food approved by the US FDA, the European Council and the Food Spice Expert Committee and the Chinese Ministry of Health. The aromas of the carvone enantiomers are significantly different, with D-carvone having a coriander aroma and L-carvone having a spearmint aroma. [0003] Spearmint oil is a colorless to light yellow-green clear oily liquid with a special aroma of spearmint leaves and a sweet taste, which can produce special flavors in food. It can be used as an ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 鲍会梅
Owner 淮安琼林科技有限公司
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