Method for capturing cells or biomolecules in solutions

Abstract

The invention discloses a method for capturing cells or biomolecules. The method comprises the following steps: (I) enabling a medium containing target cells or biomolecules to enter a device comprising a capturing mechanism for capturing the target cells or biomolecules; (II) enabling the medium to flow through the capturing mechanism so that the target cells or biomolecules are bound to the capturing structure; (III) removing the needless impurities, cells or molecules which are not specifically bound to the capturing mechanism; (IV) enabling the target cells or biomolecules to be separated from the capturing mechanism; and (V) collecting the target cells or biomolecules. The capturing mechanism comprises one or more stacked capturing sieves, wherein each capturing sieve comprises a net-shaped base body and a capturing layer formed on the net-shaped base body, and the capturing layer contains capturing matter which can be in specific binding with the target cells or biomolecules. The method disclosed by the invention has high specificity and high flux, is suitable for capturing the cells or capturing the biomolecules in the solutions by adopting molecules expressed by cells, and is particularly suitable for capturing and sorting circulating tumor cells.

Description

technical field [0001] The present invention relates to a device for capturing a cell or capturing a biomolecule in solution by a biomolecule expressed by the cell, and more particularly to a device for capturing a target cell (eg, circulating tumor cells) or A capture screen for capturing target biomolecules in a solution and a device having such a capture screen. Background technique [0002] Circulating tumor cells, that is, tumor cells circulating in the blood, are considered to have a significant relationship with distant metastasis of tumors and other issues. Generally, there are only 1-10 circulating tumor cells in 10ml blood of cancer patients, and their slow capture speed or poor specificity is an urgent problem to be solved for rapid detection of patient blood samples. [0003] Current sorting methods such as the traditional immunomagnetic bead sorting method have good repeatability, high sensitivity, and good specificity, and can quantitatively analyze circulatin...

AI Technical Summary

Problems solved by technology

Generally, there are only 1-10 circulating tumor cells in 10ml blood of cancer patients, and their slow capture speed or poor specificity is an urgent problem to be solved for rapid detection of patient blood samples

[0003] The current sorting methods such as the traditional immunomagnetic bead sorting method have good repeatability, high sensitivity, good specificity, and can quantitatively analyze circulating tumor cells, but the operation speed is slow and the throughput is small

Although membrane microfiltration technology and gradient density centrifugation method are simple to operate and have good cell viability after separation, they have low specificity and high false positive rate

Microfluidic technology is simple to operate and requires a small amount of antibodies, but it is costly and has a high false negative rate

Moreover, microfluidic technology companies are now mainly targeting scientific research customers, who need to mix multiple reagents in advance, rely too much on old-fashioned chips, and have difficulties in industrialization, so it is difficult to enter the field of in vitro diagnostics

Others, such as the Cell Search system in the United States, have high sensitivity and high specificity, but consume a lot of blood, require a large amount of antibodies, and are expensive, and cannot capture living cells and collect and re-culture circulating tumor cells, so they can only do DNA sequencing cannot be used for RNA sequencing, and cannot be used as a medication guide

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