Blood platelet LncRNA quantitative determination method for non-small cell lung cancer diagnosis
A non-small cell lung cancer, platelet technology, applied in the field of tumor molecular biology, can solve the problem of accurate diagnosis without tumor, achieve high diagnostic efficiency and sensitivity, far-reaching clinical significance and promotion effect
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[0032] Platelets from 101 NSCLC (68 adenocarcinoma + 33 squamous cell carcinoma) tumor patients and 60 healthy controls were used to detect MAGI2-AS3 and ZFAS1 and establish models. The workflow is shown in figure 1 as shown,
[0033] The specific detection method includes the following steps:
[0034] 1. Collection and storage of platelets
[0035] The venous blood of the subjects was collected in 4ml anticoagulant tubes, and the platelets were centrifuged within 12 hours at room temperature. First, centrifuge at 120g at room temperature for 20min, divide into two layers, absorb the platelet-rich plasma from the upper layer, and transfer it to a new EP tube (pay attention to keeping part of the plasma when absorbing, not to absorb all), centrifuge at 360g for 20min, the precipitate is platelets, and wash two times with PBS. The second time, add 300μl DEPC water and extract RNA.
[0036] 2. Platelet RNA extraction (blood total RNA rapid extraction kit, BioTeKe)
[0037] 1)...
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