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A Bacillus subtilis strain and its application

A Bacillus subtilis, bacterial liquid technology, applied in the field of applied biology, can solve the problems of secondary air pollution, skin allergies, poor breathing, etc., and achieves stable deodorization effect, convenient transportation, and growth-promoting effects.

Active Publication Date: 2021-06-18
CHENGDU YOUYIJIA ENVIRONMENTAL PROTECTION EQUIP ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these chemical fungicides are often accompanied by pungent odors, which will cause secondary pollution of the air quality in the toilet. Improper spraying will also cause poor breathing and skin allergies.
Moreover, because the chemical composition of the fungicide will volatilize after contact with the air, it needs to be sprayed several times in the same toilet from time to time to achieve the desired effect, which brings certain safety hazards to the spraying personnel and toilet users.

Method used

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  • A Bacillus subtilis strain and its application
  • A Bacillus subtilis strain and its application
  • A Bacillus subtilis strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Breeding of Bacillus subtilis CGMCC13714

[0029] (1) Suspend 10 mL of aquaculture sewage discharged from a pig farm in Shuangliu, Sichuan, in 90 mL of sterile distilled water, shake evenly, boil for 5 minutes, and perform gradient dilution after cooling, respectively starting from a dilution of 10 -2 、10 -4 、10 -6 、10 -8 、10 -10 0.1ml of the diluted solution was drawn, spread on the screening medium, and cultured at 35°C for 24 hours to obtain multiple colonies. The screening medium used here is beef extract peptone medium, its components include: peptone 10g, beef extract 3g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4, after 121℃, 30min high temperature sterilization .

[0030] (2) Select 5 bacterial colonies with different shapes, vigorous growth, smooth surface and sticky surface. Under the same culture conditions, these 5 bacterial strains are repeatedly streaked and cultured for more than three times to obtain pure bacterial strains, w...

Embodiment 2

[0035] Example 2: Application of Bacillus subtilis CGMCC13714 in feces deodorization

[0036] 1. Preparation of culture medium and inoculation solution

[0037] Solid activated medium: peptone 10g, beef extract 10g, yeast extract 5g, sodium chloride 5.0g, agar 15g, add water to 1000ml, pH7.0~7.4. Liquid expansion fermentation medium: peptone 5g, beef extract 5g, glucose 10g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4.

[0038] Inoculate Bacillus subtilis CGMCC13714 into the activation medium for activation and culture for 24 hours to obtain activated strains for use; inoculate the activated strains for expanded fermentation medium, shake and culture for 24 hours at 35°C and 120rmp to obtain an inoculum liquid for use.

[0039] 2. Feces deodorization treatment

[0040]Inoculate 5ml of the inoculum solution into a 1L beaker containing 500g of human feces (inoculum size 1.0%, V / W), and seal the beaker with plastic wrap; do not add the control of the bacterium solution...

Embodiment 3

[0045] Example 3: Application of Bacillus subtilis CGMCC13714 in feces deodorization

[0046] 1. Preparation of culture medium and inoculation solution

[0047] Activation medium: peptone 10g, beef extract 10g, yeast extract 5g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4. Liquid expansion fermentation medium: peptone 5g, beef extract 5g, glucose 10g, sodium chloride 5.0g, add water to 1000ml, pH7.0~7.4.

[0048] Inoculate Bacillus subtilis CGMCC13714 into the activation medium for activation and culture for 24 hours to obtain activated strains for use; inoculate the activated strains for expanded fermentation medium, shake and culture for 24 hours at 30°C and 120rmp to obtain an inoculum liquid for use.

[0049] 2. Feces deodorization treatment

[0050] Inoculate 5ml of the inoculum solution into a 1L beaker containing 500g of human feces (inoculum size 1.0%, V / W), and seal the beaker with plastic wrap; do not add the control of the bacterium solution at the same t...

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Abstract

The invention relates to the field of applied biotechnology. The purpose is to provide a strain of Bacillus subtilis and its application in the preparation of deodorant bacteria. The preservation number of the bacterial strain in the General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms is: CGMCC No.13714. The technical scheme adopted is: inoculate Bacillus subtilis CGMCC13714 in the activated medium and cultivate to obtain the activated strain, inoculate the activated strain into the expanded fermentation medium to expand the culture to obtain the bacterial liquid; inoculate the bacterial liquid in the feces and keep the temperature 20~35℃. The invention provides a new Bacillus subtilis, which can be applied to the biological deodorization of feces, and the deodorization effect of single use is stable, and is less affected by temperature; it can effectively deodorize through the in-situ deodorization method, and the operation is simple It is easy to operate, does not require supporting equipment and technology, and has a small investment scale.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, and in particular relates to a bacillus subtilis strain and an application thereof. Background technique [0002] With the rapid development of my country's economy and the improvement of people's living standards, people have higher requirements for the environment of public toilets. In addition to daily manual cleaning, odor pollution has also become an important issue in the environmental governance of public toilets. Common sterilization methods include physical sterilization and deodorization, chemical sterilization and deodorization and biological antibacterial deodorization. [0003] For the public toilet environment, it is difficult to use physical sterilization and deodorization methods to achieve sanitation and cleanliness, so chemical sterilization and deodorization are mostly used at present, such as sodium dichloroisocyanurate, calcium hypochlorite, peracetic acid, glutaraldehyde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/125
CPCC02F11/02C12N1/20C12N1/205C12R2001/125
Inventor 冯正远闫志英冯正伟
Owner CHENGDU YOUYIJIA ENVIRONMENTAL PROTECTION EQUIP ENG
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