Peptide regulating sebum secretion and cosmetics containing same
A technology of sebum secretion and cosmetics, applied in the field of non-natural peptides, can solve problems such as dry and cracked skin, affecting patients' work and life, and drug resistance
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Embodiment 1
[0064] The test of peptide of the present invention inhibiting sebum secretion
[0065] 1. Isolation and primary culture of human sebocytes
[0066] Fresh head skin samples of normal people were washed once with PBS and 70% alcohol in sequence, and placed in In culture medium (plus 2% fetal bovine serum, antibiotic mixture). Subcutaneous fat was removed, the skin was cut into 0.5cm×0.5cm size, neutral protease was added, sealed and placed in a refrigerator at 4°C overnight. Peel off the epidermis under a stereo microscope, separate more than 15 sebaceous glands with tweezers, and place them in a collagen-coated cell culture dish (a cell culture dish with a diameter of 6 cm) The culture medium is not more than 1.5mL), placed in a 5% CO2 cell incubator, and cultured at 37°C for 2-3 days. After the sebocytes are fixed, replace Culture medium (add 10% fetal bovine serum, 5 μg / L recombinant human EGF, and antibiotic mixture to each bottle of culture medium), culture in a 5% ...
Embodiment 2
[0074] Embodiment 2: the preparation of blank cosmetics
[0075]
[0076]
Embodiment 3
[0077] Embodiment 3: the preparation of cosmetics
[0078] Element Content (weight%) Peptide of the invention (SEQ ID NO: 1) 0.001 Retinol 3.0 Forsythia Fruit Extract 1.5 Danshen Extract 1.5 Anemarrhena Extract 3.0 White Willow Bark Extract 5.0 1,3 propanediol 3.0 Sophora flavescens extract 5.0 Soy Lecithin 3.0 Dextran 3.0 Nicotinamide 1.0 ethyl lauroyl arginate 3.0 purified water Residual amount total 100
[0079] Dissolve dextran in purified water, add 1,3 propylene glycol, stir evenly, add other components, stir and prepare a beauty cream.
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