A kind of lyophilized liquid formula and its application
A freeze-drying and formulating technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of difficulty in meeting the preservation requirements of the kit, use restrictions, and different preservation effects, and achieve good product appearance, improved molding rate, and good freezing. dry effect
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[0021] Preparation of lyophilized solution: 1% sodium caseinate, 5% mannitol, and 0.1% polypyrrolidone K30 (PVPK30) were dissolved in 0.01 mol / L phosphate buffer (PBS) with a pH of 7.4.
[0022] The formulas of all the other embodiments and comparative examples are shown in Table 1
[0023] Table 1
[0024]
[0025] The preparation of monkey cytokine IL-12 / 23 ELISA standard product comprises the following steps:
[0026] S1. Pre-cool freeze-drying related equipment, such as vials, racks, and trays, to 4°C;
[0027] S2. After the monkey cytokine IL-12 / 23 standard protein was dissolved in water, it was prepared into a mother liquor, and the mother liquor was diluted to the target concentration with the lyophilized solution of each embodiment or comparative example to obtain a standard concentration protein solution;
[0028] S3. Dispense the standard concentration protein solution obtained in S2. into S1. pre-cooled 3mL vials, fill each bottle with 1mL protein solution, and...
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