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Uses of poria cocos extract and tumulosic acid in protecting muscles

A Poria cocos extract and extract technology, applied in the field of muscle protection of Poria cocos extract and turmoic acid, can solve problems such as no effective treatment or delay of muscle atrophy

Active Publication Date: 2017-10-27
SINPHAR TIAN LI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there is no effective treatment or method to delay muscle atrophy clinically

Method used

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  • Uses of poria cocos extract and tumulosic acid in protecting muscles
  • Uses of poria cocos extract and tumulosic acid in protecting muscles
  • Uses of poria cocos extract and tumulosic acid in protecting muscles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation and component analysis of Poria cocos extract

[0043] (1-1)

[0044] Take the medicinal material of Poria cocos (the place of origin is Yunnan), wash and peel off its outer skin (hereinafter referred to as "Poria cocos skin part"), and the rest is the meat part (hereinafter referred to as "Poria cocos meat part").

[0045] (1-2)

[0046] Get 700 kilograms of Poria cocos meat part of (1-1), soak in 75% ethanol with the volume ratio of about 1:8 (Poria cocos meat part: 75% ethanol) at room temperature, last 12 hours, then boil and carry out extraction (duration 3 hours). The aforementioned extraction steps were repeated three times in total. The extracts from the three extractions were combined and filtered to remove insolubles. Then, concentrate under reduced pressure to remove ethanol, and then dry with a spray dryer to obtain an extract.

[0047] (1-3)

[0048]With liquid chromatography / ultraviolet light / mass spectrometry (LC / UV / MS, liquid...

Embodiment 2

[0059] Example 2: Establishment of muscle cell injury model

[0060] Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine (cytokine) with a molecular weight of 17,000. Human clinical data show that in some special diseases (such as cancer, AIDS, chronic obstructive pulmonary disease, etc.) muscle breakdown) or increased muscle cell death. Studies have shown that injecting TNF-α into experimental animals or administering drugs to increase the concentration of TNF-α in experimental animals will induce muscle cell damage (including muscle protein metabolism imbalance, muscle cell death, etc.), and then lead to muscle atrophy. In order to investigate the efficacy and mechanism of Poria cocos extract and its components in protecting muscle, the inventor induced TNF-α to establish a muscle cell injury model.

[0061] First, C2C12 cells (i.e., mouse myoblasts, purchased from ATCC) were cultured with H-DMEM medium (purchased from Sigma Company), until the C2C12 cells g...

Embodiment 3

[0063] Example 3: Effect of Poria cocos extract on anti-muscle cell damage

[0064] The Poria cocos extract provided in Example 1 (1-4) was used to prepare a Poria cocos meat extract solution with dimethyl sulfoxide (DMSO; purchased from Sigma). After the C2C12 cells grew to 80% confluence in H-DMEM medium, they were divided into five groups and processed as follows:

[0065] (1) Control group: cultured in H-DMEM medium (without Poria cocos extract) for 6 hours. Next, the culture medium was replaced with a differentiation medium of 2% horse serum for 4 days.

[0066] (2) TNF-α group: cultured in H-DMEM medium for 6 hours. Next, the medium was replaced with 2% horse serum differentiation medium, and TNF-α was added to the medium at a final concentration of 10 ng / ml for co-treatment with the differentiation medium for 4 days.

[0067] (3) TNF-α + Poria cocos extract group (three groups in total): add the above-mentioned Poria cocos meat extract solution to the H-DMEM medium, ...

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Abstract

Uses of a poria cocos extract, a tumulosic acid and / or a pharmaceutically acceptable salt of the tumulosic acid in the preparation of a drug or food, and the drug or the food is used for protecting muscles. The drug is especially used for resisting against muscle cell damages and promoting the regeneration and recovery of muscles, so as to adjust, treat and / or delay muscle atrophies, and especially muscle atrophies caused by aging, diseases and / or cachexia. The food is a health food, a nutrient supplementary food or a special nutritional food.

Description

technical field [0001] The present invention relates to the application of Poria cocos (FU-LING (Poria cocos)) extract and tumulosic acid (tumulosic acid) (ie, a component of Poria cocos extract) or a pharmaceutically acceptable salt of tumulosic acid, especially about Use of Poria cocos extract, turmoic acid, or a pharmaceutically acceptable salt of turmoic acid to protect muscles, including anti-muscle cell damage, promoting muscle regeneration and repair, to regulate, treat and / or delay muscle atrophy, Especially muscle wasting due to aging, disease, and / or cachexia. Background technique [0002] Muscle tissue is the highest quality tissue in mammals, and its main function is to generate force to pull body parts in motion. Muscle can be divided into three types: skeletal muscle, cardiac muscle and smooth muscle. Skeletal muscle can be further divided into slow muscle and fast muscle according to its own metabolic type and characteristics. The former is composed of slow ...

Claims

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Application Information

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IPC IPC(8): A61K36/076A61K31/575A61P21/00A23L33/10
CPCA23L33/10A61K31/575A61K36/076A23V2002/00A23V2250/208A23V2200/30A61K2236/00A61P21/00A61K31/19A23V2200/316
Inventor 王昭日郭汉鹏叶艾灵
Owner SINPHAR TIAN LI PHARMA
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