A rapid and accurate method for identifying fragrant and non-fragrant plants in the early growth stage of rice

Abstract

The invention relates to a method for identifying the variety of a plant, and particularly relates to a method for quickly and accurately identifying aromatic and non-aromatic plantlets at the early growth period of paddy rice. The method comprises the following steps of a step 1, preparation of a sample: grinding a blade at the heading initial period of the paddy rice by using liquid nitrogen, weighing 20mg to 50mg of the amount of the sample, and putting into a headspace sample bottle for later use; a step 2, making the sample enter an automatic solid-phase micro-extraction stage, wherein the extraction is divided into three stages of 1), a first stage: the preheating is maintained for 2 to 5 minutes at 80 DEG C; 2), a second stage: the step that an extraction head is at an interval of shaking for 20 seconds and stopping for 5 seconds at 80 DEG C and at the rotational speed of 250rpm / min is adopted, the depth at which the extraction head is inserted into a sample feeding bottle is 22 millimeters, and the extraction stage lasts for 10 to 15 minutes; 3), a third stage: an extract is enabled to directly enter to a sample feeding opening of a gas chromatograph-mass spectrometer; the sample feeding time is 1 minute; the sampling feeding temperature is 260 DEG C; a step 3, making the sample enter to the chromatograph-mass spectrometer, and carrying out analysis.

Description

technical field [0001] The invention relates to a method for identifying plant varieties, in particular to a method for quickly and accurately identifying fragrant and non-fragrant plants in the early growth stage of rice. Background technique [0002] Fragrant rice is loved by domestic and foreign consumers for its delicate fragrance and delicious taste. The price of high-quality fragrant rice in the market is generally 2 to 3 times that of ordinary rice. Aromatic rice is rich in resources and has a long history of cultivation, almost all over the world's rice-producing countries, India, Thailand, Pakistan, Vietnam, Japan and the United States all have their own high-quality aromatic rice varieties; such as India's Basmati fragrant rice (Basmati) , Thailand's Khao Dawk Mail 105, Australia's Goolarah, America's Della, Iran's Sadri, Japan's Congratulations, and China's Wannian fragrant rice are all famous varieties of fragrant rice. Numerous breeders in China now attach grea...

AI Technical Summary

Problems solved by technology

Instrumental analysis methods, but currently applied to rice aroma collection technologies, such as supercritical fluid extraction, headspace gas trapping, simultaneous distillation extraction and column adsorption, etc., have the disadvantages of large consumption of samples and reagents, complicated operation steps and time-consuming collection and concentration and other shortcomings, it is difficult to meet the identification work of large-scale test materials in breeding work

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