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Method and device for fluorescence detection of label-free micro-nano particles

A fluorescent detection and label-free technology, applied in the field of near-field optics, can solve the problems of difficult probe screening and direct detection

Active Publication Date: 2020-10-20
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the small size of such substances, it is difficult to directly detect them. Fluorescent labeling is usually used. This method has outstanding advantages in detection sensitivity, but it needs to screen out specific molecular probes. When the particle to be detected is unknown When , the screening of probes is more difficult

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  • Method and device for fluorescence detection of label-free micro-nano particles

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Embodiment

[0015] A method and device for detecting fluorescence of polystyrene particles with a label-free diameter of 60nm, characterized in that the surface plasmon resonance of noble metals is excited by using a total internal reflection structure, and the micro-nano particles on the surface of the noble metal film interact with their surface plasmon resonances and are affected Suppressing total internal reflection, the Fr633 (sigma company) fluorescent substance with an absorption peak of 633nm in the solution around the particles is excited by its transmitted light to emit light. The method is applied to the following device, which includes: a laser 1, a polarization adjuster 2, and Prism 3 for matching optical wavevector-plasma wavevector on metal film surface, noble metal film plated on glass slide 4, bottomless sample chamber 5, fluorescence collecting lens 6, optical neutral filter 7, photodetector 8, processing Circuit 9, computer 10, and rotating table 11; wherein, the light e...

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Abstract

The invention discloses a fluorescent detection method for unmarked micro-nano particles and a device and belongs to the technical field of near-field optics. The fluorescent detection method is characterized that surface plasma resonance of a noble metal is stimulated by using a total-internal-reflection structure, micro-nano particles on the surface of a noble metal film are subjected to frustrated total-internal-reflection after resonant interaction with surface plasma of the noble metal film, and a fluorescent solution in which the particles are located is stimulated through transmission light of the particles to emit light. The device comprises a laser device, a polarization adjuster, an optical wave vector-metal film surface plasma coupler, a glass slide coated with the noble metal film at one side, a bottomless sample tank, a fluorescence collecting lens, an optical neutral filter, a photoelectric detector, a processing circuit, a computer and a rotating platform. Unmarked direct detection of the micro-nano particles is achieved, and the concentration of the particles is directly represented through the fluorescence intensity.

Description

technical field [0001] The invention belongs to the technical field of near-field optics, and relates to the effect of micro-nano structures and evanescent fields, in particular to a detection method and device for unmarked micro-nano particles by exciting fluorescence through frustrated total internal reflection. Background technique [0002] The detection of micro-nano particles is very important in the field of biomedicine. For example, the typical size range of viruses is between 20nm-400nm. The accurate detection of such substances can provide important detection for plague forecasting, plague outbreak control and public safety protection. means. However, due to the small size of such substances, it is difficult to directly detect them. Fluorescent labeling is usually used. This method has outstanding advantages in detection sensitivity, but it needs to screen out specific molecular probes. When the particle to be detected is unknown When , the screening of probes is m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/648
Inventor 洪昕
Owner DALIAN UNIV OF TECH
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