Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of vanillin in preparation of drugs for treating/preventing bone matabolic diseases

A technology of vanillin and bone metabolism, applied in the field of biomedicine, to achieve the effect of inhibiting expression, less toxic and side effects, and promoting apoptosis

Active Publication Date: 2017-10-24
ARMY MEDICAL UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report or application of vanillin in the skeletal system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of vanillin in preparation of drugs for treating/preventing bone matabolic diseases
  • Application of vanillin in preparation of drugs for treating/preventing bone matabolic diseases
  • Application of vanillin in preparation of drugs for treating/preventing bone matabolic diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1, the effect of vanillin concentration on inhibiting the proliferation of osteoclast precursors

[0032] RAW264.7 cells were treated with 1×10 3cells / well were inoculated in 96-well plates, and the medium was discarded after the cells were overgrown. Add 0mM, 0.0625mM, 0.125mM, 0.25mM, 0.5mM, 1mM, 2mM, 4mM vanillin into each well plate containing fresh medium (DMSO concentration is controlled at less than 0.1%) to intervene RAW264.7 cells . Cultured for 24h, 48h, and 72h respectively, the cell activity was detected by the CCK-8 method, and the effect of vanillin on the proliferation of RAW264. A step-by-step experimental investigation.

[0033] The result is as figure 1 shown, where figure 1 A is the chemical formula of vanillin; figure 1 Middle B-D are graphs showing the effect of vanillin on the proliferation of RAW264.7 cells, the abscissa is the dosage of vanillin at different concentrations, and the ordinate is the 450nm absorbance OD value of each g...

Embodiment 2

[0035] Example 2, vanillin concentration-dependent reduction of RANKL-induced osteoclast (TRAP positive cells) formation

[0036] No RANKL induction was used as the control group, and RANKL induction with different vanillin concentrations was used as the experimental group according to the following conditions:

[0037] Group 1: no RANKL induction, 0mM vanillin control group;

[0038] Group 2: RANKL (50ng / mL) induction, 0mM vanillin experimental group;

[0039] Group 3: RANKL (50ng / mL) induction, 0.25mM vanillin experimental group;

[0040] Group 4: RANKL (50ng / mL) induction, 0.5mM vanillin experimental group;

[0041] Group 5: RANKL (50ng / mL) induction, 1mM vanillin experimental group;

[0042] Group 6: RANKL (50ng / mL) induction, 2mM vanillin experimental group.

[0043] RAW264.7 cells were treated with 2×10 3 cells / well were inoculated in 96-well plates, and the medium was discarded after the cells were overgrown. Add vanillin at 0, 0.25mM, 0.5mM, 1mM, 2mM to each well...

Embodiment 3

[0045] Example 3, Vanillin Concentration-Dependently Reduces the Formation of Multinucleated Mature Osteoclasts Induced by RANKL

[0046] No RANKL induction was used as the control group, and RANKL induction with different vanillin concentrations was used as the experimental group according to the following conditions:

[0047] Group 1: no RANKL induction, 0mM vanillin control group;

[0048] Group 2: RANKL (50ng / mL) induction, 0mM vanillin experimental group;

[0049] Group 3: RANKL (50ng / mL) induction, 0.25mM vanillin experimental group;

[0050] Group 4: RANKL (50ng / mL) induction, 0.5mM vanillin experimental group;

[0051] Group 5: RANKL (50ng / mL) induction, 1mM vanillin experimental group;

[0052] Group 6: RANKL (50ng / mL) induction, 2mM vanillin experimental group.

[0053] The RAW264.7 subcultured cells were continued to be cultured, and after the cell density at the bottom of the bottle reached 90%, the cells were scraped off with a cell scraper to collect the cell...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of vanillin in preparation of drugs for treating / preventing bone matabolic diseases. The vanillin is used for inhibiting the propagation of osteoclasts, the formation of polynuclear mature osteoclasts and the bone crushing capability of the osteoclasts, promoting the apoptosis of mature osteoclasts and regulating and controlling differentiation, fusion and maturing genes and apoptosis related genes of the osteoclasts and has the effects of maintaining a bone stable state, preventing bone loss and maintaining bone density, and novel drugs are provide for treating / preventing osteoclast activity induced diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of vanillin in the preparation of medicines for treating / preventing bone metabolism diseases. Background technique [0002] Osteoclasts (OCs) are derived from monocyte / macrophage cell lines differentiated from hematopoietic stem cells (HSCs), which are quite different from the rest of the bone cells in the bone. The specificity of its source determines the multifaceted role of osteoclasts in the skeletal system. In addition to its main function of bone resorption, osteoclasts play an important role in angiogenesis, osteoimmunology and hematopoiesis. Both play an important role in the formation of the microenvironment (hematpoietic niche). However, the most important and most studied function of osteoclasts is their ability to resorb bone and their interaction with the rest of the osteoblast-dominated bone cells. Bone resorption by osteoclasts and bone regeneration by ost...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/11A61P19/10A61P19/08
CPCA61K31/11
Inventor 陈玥琦董世武窦策
Owner ARMY MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com