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Mass spectrometer substrate target holder and apparatus for biomarker detection

A technology of biomarkers and substrates, which is applied in the direction of mass spectrometers, microbiological determination/inspection, biochemical equipment and methods, etc., can solve the problem of single application field, simultaneous injection of multiple target plates, low identification accuracy, etc. problem, saving time

Active Publication Date: 2017-10-20
BIOYONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The traditional MALDI-TOF MS target plate has a single application field, such as the MALDI-TOF Axima produced by SHIMADZU, the target plate is only used for protein microbial identification; the MALDI-TOF MASSARRY produced by AGENA is only used for genetic detection; there is no such MALDI-TOF mass spectrometry target plate covering three applications of gene detection and protein microbial identification
[0008] In addition, the throughput of traditional MALDI-TOF target plates is fixed, the throughput of protein microbial substrates is 384well, 48well, and the throughput of gene substrates is 384well, 96well, 24well. In the same way, multiple experimenters cannot conduct experiments at the same time, the mass detection accuracy of mass spectrometry is low, and the identification accuracy is low, etc.

Method used

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  • Mass spectrometer substrate target holder and apparatus for biomarker detection
  • Mass spectrometer substrate target holder and apparatus for biomarker detection
  • Mass spectrometer substrate target holder and apparatus for biomarker detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. Influence of flatness of target support on the accuracy of identification results

[0033] Taking the deafness gene standard product as an example, there are 18 mass spectrum peaks in total, and the mass range is 3000-9000Da. Three types of flatness of 10μm, 20μm, and 30μm are selected, and 12 replicates of the same sample are made for each flatness, and the results of each peak are analyzed and compared. Mass deviation, calculates mass accuracy.

[0034] parameter settings:

[0035] Turing mode: linear

[0036] Mass Range: 3000-9000

[0037] Max Laser Rep Rate: 10.0

[0038] Power: 90

[0039] Profiles: 40

[0040] Shots: 10

[0041] 1) The flatness of the groove of the substrate is less than 10 μm, 12 repetitions, and the peak deviation of each position: (gray is the maximum deviation)

[0042]

[0043] The maximum deviation is at the position of 8214.4Da, the deviation is 2.78Da, and the mass accuracy is less than 350ppm.

[0044] 2) The flat...

Embodiment 2

[0051] Embodiment two, microbial identification

[0052] Select 5 fresh strains of microorganisms, 3 replicates for each strain, the mass range is 2000-20000Da, the collected data is scored in BE3.0 software, and the confidence results are checked.

[0053] parameter settings:

[0054] Turing mode: linear

[0055] Mass Range: 2000-20000

[0056] Max Laser Rep Rate: 30.0

[0057] Power: 60

[0058] Profiles: 100

[0059] Shots: 5

[0060]

[0061]

[0062] Spectrum see Figure 4 Spectrum of microbiological detection Escherichia coli.

[0063] Conclusion: Confidence > 25 points means that the identification results are credible, and the identification results of the 15 sets of data in this experiment are all correct, with a correct rate of 100%.

Embodiment 3

[0064] Embodiment 3, gene detection

[0065] Select deafness plasmid samples, a total of 20 mass spectrum peaks, mass range 3000-9000Da, 5 replicates of positive samples, 5 replicates of negative samples, and analyze the identification accuracy of the test results.

[0066] parameter settings:

[0067] Turing mode: linear

[0068] Mass Range: 3000-9000

[0069] Max Laser Rep Rate: 10.0

[0070] Power: 90

[0071] Profiles: 40

[0072] Shots: 10

[0073] Deafness plasmid sample identification results:

[0074]

[0075]

[0076]

[0077]

[0078]

[0079]

[0080] Spectrum see Figure 5 Plasmid spectrum for genetic testing of deafness.

[0081] Conclusion: 10 deafness plasmid samples were detected based on the flatness <10 μm target plate, and each deafness plasmid had 20 loci, a total of 200 loci, and the identification result accuracy rate was 100%.

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Abstract

The invention provides a mass spectrometer substrate target holder for biomarker detection. The mass spectrometer substrate target holder comprises a directional guide angle, a positioning slot, a gene substrate slot, a protein microbiological substrate slot, a mass spectrometry substrate slot first step, a mass spectrometry substrate slot second step, a mass spectrometry substrate slot third step and a mass spectrometry substrate slot through hole. The flatness of a target holder slot directly affects the mass spectrometry detection quality precision so that accuracy is influenced. The flatness of the target holder slot is less than 10 microns. The mass spectrometry detection quality precision is less than 350ppm. The accuracy of mass spectrometry identification is greatly improved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a mass spectrometry substrate target holder and an instrument for BIOMARK detection. Background technique [0002] Time-of-flight mass spectrometry began in the 1940s. Due to the backwardness of electronic technology and equipment at that time, the resolution of the instrument was less than 100, which made it difficult to popularize and apply. With the passage of time and the advancement of science and technology, by the end of the 1980s, after Hillenkamp et al. invented a new method for matrix-assisted laser desorption ionization, they combined this soft ionization method with time-of-flight mass spectrometry to form a new type of instrument: matrix-assisted Laser desorption ionization / time-of-flight mass spectrometry, namely MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry), is a new type of soft ionization organic mass spectrometry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/10C12Q1/14C12Q1/68H01J49/40
CPCC12Q1/04C12Q1/10C12Q1/14C12Q1/6872H01J49/40C12Q2563/107C12Q2565/627
Inventor 马庆伟梁飞白春雨付书辉党文娟
Owner BIOYONG TECH
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