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Multifunctional Mass Spectrometry Substrate Target Holder

A multi-functional, substrate-based technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of single application field, inability to conduct experiments at the same time, and inability to choose and match multiple applications, and achieve the effect of saving time

Active Publication Date: 2019-01-29
BIOYONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The traditional MALDI-TOF MS target plate has a single application field, such as the MALDI-TOF Axima produced by SHIMADZU, the target plate is only used for protein microbial identification; the MALDI-TOF MASSARRY produced by AGENA is only used for genetic detection; there is no such MALDI-TOF mass spectrometry target plate covering three applications of gene detection and protein microbial identification
[0008] In addition, the throughput of traditional MALDI-TOF target plates is fixed, the throughput of protein microbial substrates is 384well, 48well, and the throughput of gene substrates is 384well, 96well, 24well. In the same way, multiple experimenters cannot conduct experiments at the same time, the mass detection accuracy of mass spectrometry is low, and the identification accuracy is low, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. Influence of flatness of target support on the accuracy of identification results

[0033] Taking the deafness gene standard product as an example, there are 18 mass spectrum peaks in total, and the mass range is 3000-9000Da. Three types of flatness of 10μm, 20μm, and 30μm are selected, and 12 replicates of the same sample are made for each flatness, and the results of each peak are analyzed and compared. Mass deviation, calculates mass accuracy.

[0034] parameter settings:

[0035] Turing mode: linear

[0036] Mass Range: 3000-9000

[0037] Max Laser Rep Rate: 10.0

[0038] Power: 90

[0039] Profiles: 40

[0040] Shots: 10

[0041] 1) The flatness of the groove of the substrate is less than 10 μm, 12 repetitions, and the peak deviation of each position: (gray is the maximum deviation)

[0042]

[0043] The maximum deviation is at the position of 8214.4Da, the deviation is 2.78Da, and the mass accuracy is less than 350ppm.

[0044]2) The flatn...

Embodiment 2

[0051] Embodiment two, microbial identification

[0052] Select 5 fresh strains of microorganisms, 3 replicates for each strain, the mass range is 2000-20000Da, the collected data is scored in BE3.0 software, and the confidence results are checked.

[0053] parameter settings:

[0054] Turing mode: linear

[0055] Mass Range: 2000-20000

[0056] Max Laser Rep Rate: 30.0

[0057] Power: 60

[0058] Profiles: 100

[0059] Shots: 5

[0060]

[0061]

[0062] Spectrum see Figure 4 Spectrum of microbiological detection Escherichia coli.

[0063] Conclusion: Confidence > 25 points means that the identification results are credible, and the identification results of the 15 sets of data in this experiment are all correct, with a correct rate of 100%.

Embodiment 3

[0064] Embodiment 3, gene detection

[0065] Select deafness plasmid samples, a total of 20 mass spectrum peaks, mass range 3000-9000Da, 5 replicates of positive samples, 5 replicates of negative samples, and analyze the identification accuracy of the test results.

[0066] parameter settings:

[0067] Turing mode: linear

[0068] Mass Range: 3000-9000

[0069] Max Laser Rep Rate: 10.0

[0070] Power: 90

[0071] Profiles: 40

[0072] Shots: 10

[0073] Deafness plasmid sample identification results:

[0074]

[0075]

[0076]

[0077]

[0078]

[0079]

[0080] Spectrum see Figure 5 Plasmid spectrum for genetic testing of deafness.

[0081] Conclusion: 10 samples of deafness plasmids were detected based on the flatness < 10 μm target plate, and each deafness plasmid had 20 loci, a total of 200 loci, and the identification result accuracy rate was 100%.

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Abstract

The invention provides a multifunctional mass spectrometry substrate target holder. The multifunctional mass spectrometry substrate target holder comprises a main target holder body as well as gene substrate slots and protein microorganism substrate slots formed in the main target holder body, wherein each of the gene substrate slots and the protein microorganism substrate slots is of a square structure, and slot through holes are formed in four corners of the square structure respectively. The flatness of each slot of the target holder directly affects quality precision of mass spectrometric detection and further affects the accuracy, the flatness of each slot of the target holder is smaller than 10 mu m, the quality precision of mass spectrometric detection is lower than 350 ppm, and the quality precision of mass spectrometry identification accuracy is improved greatly.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a multifunctional mass spectrometry substrate target holder for BIOMARK detection. Background technique [0002] Time-of-flight mass spectrometry began in the 1940s. Due to the backwardness of electronic technology and equipment at that time, the resolution of the instrument was less than 100, which made it difficult to popularize and apply. With the passage of time and the advancement of science and technology, by the end of the 1980s, after Hillenkamp et al. invented a new method for matrix-assisted laser desorption ionization, they combined this soft ionization method with time-of-flight mass spectrometry to form a new type of instrument: matrix-assisted Laser desorption ionization / time-of-flight mass spectrometry, namely MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry), is a new type of soft ionization organic mass spectrometry de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6848
Inventor 马庆伟梁飞白春雨梁坤党文娟
Owner BIOYONG TECH
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