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Method for detecting toxicity of perfluorooctane sulfonate in water by utilizing selenastrum capricornutum

A technology of perfluorooctane sulfonic acid and crescent moon algae, which is applied in the detection field, can solve the problems of wasting resources, long test period and the like, and achieves the effects of low cost and convenient method

Inactive Publication Date: 2017-10-10
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological growth cycle of these models and non-models is more than two weeks, the test cycle is long, and resources are wasted

Method used

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  • Method for detecting toxicity of perfluorooctane sulfonate in water by utilizing selenastrum capricornutum
  • Method for detecting toxicity of perfluorooctane sulfonate in water by utilizing selenastrum capricornutum
  • Method for detecting toxicity of perfluorooctane sulfonate in water by utilizing selenastrum capricornutum

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Experimental program
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Effect test

Embodiment 1

[0022] The method for detecting the toxicity of perfluorooctane sulfonic acid in water by using Crescent algae of the present invention comprises the following steps:

[0023] 1) Cultivation of Crescent algae

[0024] Crescent algae is cultured in L9 medium, transferred once every 2 to 4 days, and transferred at least three times in a row to ensure that it is in the logarithmic growth phase, and cultivated under 23±1°C and 4440-8880lux continuous light conditions;

[0025] 2) Growth inhibition test

[0026] Set perfluorooctane sulfonic acid as 5 concentration gradients, respectively 2.0, 4.0, 8.0, 16.0, 32.0, 64.0 and 128.0mg / L, set 3 parallels for each gradient, and set up 1 blank control group in addition. Add 50ml of the prepared 2 times test solution concentration and 50ml of algae solution with a concentration of 4×107cells / L to each triangular flask. The test is carried out at 23±1°C under continuous light conditions, and the 72h ErC50 value is calculated using the prob...

Embodiment 2

[0036] Be that the perfluorooctane sulfonic acid (PFOA) solution of 16.0mg / L replaces the perfluorooctane sulfonic acid (PFOA) solution of 8.0mg / L in step 3 in embodiment 1 with concentration, other conditions are with embodiment 1 The test shows that compared with the control, the 16.0mg / L perfluorooctane sulfonate (PFOA) algae cell concentration increase is slightly lower than the blank control group.

Embodiment 3

[0038] Be that the perfluorooctane sulfonic acid (PFOA) solution of 32.0mg / L replaces the perfluorooctane sulfonic acid (PFOA) solution of 16.0mg / L in the step 3 in embodiment 1 with concentration, other conditions are with embodiment 1 The test showed that, compared with the control, the 32.0mg / L perfluorooctanesulfonic acid (PFOA) algae cell concentration increase was significantly greater than that of the blank control group.

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Abstract

The invention relates to the technical field of detection and specifically relates to a method for detecting toxicity of perfluorooctane sulfonate in water by utilizing selenastrum capricornutum. The method comprises the following steps: 1) culturing selenastrum capricornutum; 2) performing a growth inhibition test; 3) detecting the influences of the perfluorooctane sulfonate in different concentrations on the growth rate of selenastrum capricornutum. The method for detecting toxicity of perfluorooctane sulfonate in water by utilizing selenastrum capricornutum provided by the invention solves the problems of long period and complex operation for determining the PFOA biotoxicity in water at present; a simple, sensitive and systemic method for detecting toxicity of perfluorooctane sulfonate in water is provided; and the method is convenient, quick and low in cost.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for detecting the toxicity of perfluorooctane sulfonic acid in water by using Crescent algae. Background technique [0002] Perfluorooctanoic acid is a perfluorinated organic acid with hydrophobic and oleophobic properties. It can make fluorides have extremely high surface activity on the surface of carbon oxides, and is resistant to high temperatures and strong oxidants. Its chemical structure is far more stable than other surfactants. Therefore, it is widely used in hundreds of chemical industry, mechanical industry and daily perfluorinated chemical products. In the 1950s, it was used in the fields of manufacturing, pharmaceuticals and cosmetics, as well as electronic products and chemical coatings. Its mass production and use has caused serious environmental accumulation and persistent pollution in the global ecosystem, and has become a safety hazard that seriously...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
CPCG01N33/18
Inventor 杨鸿波龚会琴李占彬王艳娇成国坤肖飞何伟谢锋谭红
Owner BEIJING UNIV OF CHEM TECH
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