Method for producing Pu'er tea through fermentation with specially-made exogenous biological enzymes and application of method
A bio-enzyme and Pu-erh tea technology, applied in tea treatment before extraction, climate change adaptation, etc., can solve the problems of single variety and unstable quality, and achieve the effects of wide practicability, easy clean production, and simple operation of fermentation process
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Embodiment 1
[0029] Treat 100 kg of Lancang ancient tea (C.sinensis var.assamica) with hot air at 80°C for 1 hour, and spread it evenly in the sample mixing tank after cooling. ) + Aspergillus japonica (A. japonicus Saito var. japonicus. KZ1634) + Aspergillus heyangensis (A. heyangensis Qi et al. KZ1639) three single strains were suspended cultured in tea polyphenol-containing In the modified potato glucose tea polyphenol liquid medium, when the mycelium grows to a concentration of 80% V / V (mycelium / bacterial liquid), the bacterial suspension is homogenized, and then the phosphoric acid with pH7.2 The buffer solution was shaken and extracted at room temperature for 1 h (the volume ratio of the buffer solution to the cell homogenate was 2:1), centrifuged at 8000 r / min for 10 min, and the supernatant was the crude enzyme solution. Prepare the crude enzyme liquid with 0.1% glucose aqueous solution, so that the cellulase activity is 3-4U / mL, the protease activity is 4-5U / mL, and the polyphenol...
Embodiment 2
[0033] Treat 1000 kg of Lancang ecological tea (C.sinensis var.assamica) with hot air at 80°C for 1 hour, spread it evenly in the fermentation tank after cooling, and put Aspergillus stinkii (A.foetidus Thom&Raper.KZ1633)+Japan Four single strains of Aspergillus (A.japonicus Saito var.japonicus.KZ1634) + Aspergillus heyangensis (A.heyangensis Qiet al.KZ 1639) + Aspergillus proliferans (A.proliferans Smith.KZ1637) according to the ratio of 2:2:1 : 1 suspension culture in the improved potato glucose tea polyphenols liquid medium containing tea polyphenols, when the mycelium grows to a concentration of 80% V / V (mycelia / bacteria liquid), the bacterial suspension Homogenize the solution, then shake and extract with pH 7.2 phosphate buffer solution at room temperature for 1 hour (the volume ratio of the buffer solution to the cell homogenate solution is 2:1), centrifuge at 8000r / min for 10 minutes, and the supernatant is the crude enzyme liquid. Prepare the crude enzyme liquid with...
Embodiment 3
[0037] Treat 200 kg of sun-dried green hair tea made from C.makuanica with hot air at 80°C for 1 hour, and spread it evenly in the sample mixing tank after cooling. (A.foetidusThom&Raper.KZ 1633); Aspergillus japonica (A.japonicus Saito var.japonicus.KZ1634); Aspergillus grayus (A.restrictus Smith.KZ1636); Aspergillus proliferans (A.proliferans Smith.KZ1637); Seven single strains of Aspergillus (A.parasiticus Speare.KZ1638) and Aspergillus heyangensis (A.heyangensis Qi et al.KZ 1639) were inoculated according to the inoculum size of 1:1:1:1:1:1:1, and the suspension culture was in In the improved potato glucose tea polyphenol liquid medium containing tea polyphenols, when the mycelium grows to a concentration of 80% V / V (mycelium / bacterial liquid), the bacterial suspension is homogenized, and then pH7 The phosphate buffer solution of .2 was shaken and extracted at room temperature for 1 h (the volume ratio of the buffer solution to the cell homogenate was 2:1), centrifuged at ...
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