Primers, kit and method for identifying potato M viruses
An identification method, potato technology, applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc., can solve problems such as unclear functions
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Embodiment 1
[0076] A kit for identifying potato M virus RT-LAMP, the kit includes:
[0077]
[0078] Among them, the primer sequence:
[0079] PVM-F3:5'-TTCAGGCCGTGCTGTTCT-3'
[0080] PVM-B3:5'-CAGCATGTGGTTCCAAGTCA-3'
[0081] PVM-FIP:5'-GCACCCCTAGGCCACTCAAA-GGATGCAAGCAGCTCAGT-3'
[0082] PVM-BIP: 5'-GGACGCTGTGCTTGCTGTGA-CAGGCGCATACAACCTACA-3'.
Embodiment 2
[0084] A kit for identifying potato M virus RT-LAMP, the kit includes:
[0085]
[0086] Among them, the primer sequence:
[0087] PVM-F3:5'-TTCAGGCCGTGCTGTTCT-3'
[0088] PVM-B3:5'-CAGCATGTGGTTCCAAGTCA-3'
[0089] PVM-FIP:5'-GCACCCCTAGGCCACTCAAA-GGATGCAAGCAGCTCAGT-3'
[0090] PVM-BIP: 5'-GGACGCTGTGCTTGCTGTGA-CAGGCGCATACAACCTACA-3'.
[0091] The 2× reaction solution buffer described in the present invention is made up of following components:
[0092]
[0093]
[0094] The enzyme solution is a mixture of Bst DNA polymerase and AMV reverse transcriptase.
[0095] The fluorescent visual detection reagent contains calcein fluorescent dye.
Embodiment 3
[0097] A kit for identifying potato M virus RT-LAMP, the kit includes:
[0098]
[0099] Among them, the primer sequence:
[0100] PVM-F3:5'-TTCAGGCCGTGCTGTTCT-3'
[0101] PVM-B3:5'-CAGCATGTGGTTCCAAGTCA-3'
[0102] PVM-FIP:5'-GCACCCCTAGGCCACTCAAA-GGATGCAAGCAGCTCAGT-3'
[0103] PVM-BIP: 5'-GGACGCTGTGCTTGCTGTGA-CAGGCGCATACAACCTACA-3'.
[0104] The 2× reaction solution buffer described in the present invention is made up of following components:
[0105]
[0106]
[0107] The enzyme solution is a mixture of Bst DNA polymerase and AMV reverse transcriptase.
[0108] The fluorescent visual detection reagent contains calcein fluorescent dye.
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