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Rhubarb root detection method

A detection method and technology of rhubarb, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as low accuracy, affecting product production and quality assurance, and difficult product quality control.

Inactive Publication Date: 2014-01-22
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The existing technology currently has the disadvantages that the detection method is simple and backward, and the product quality is not easy to control
Existing detection methods cannot effectively control the quality of rhubarb, which will affect the production and quality assurance of products
Existing detection methods for rhubarb have poor sensitivity and low accuracy, and many medicinal materials containing rhubarb are difficult to detect. In addition, it is not known whether several species of sorrel, including Rumex batian, contain rhubarb. Ability to use it as rhubarb is unknown

Method used

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Examples

Experimental program
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Effect test

experiment example 1

[0084] Experimental Example 1 Determination of Rhein in Rhizoma Batien Rumex from Different Origins

[0085] 1 Instruments and reagents

[0086]Instruments: Agilent 1200 high performance liquid chromatography; Agilent G1315D DAD detector. Reagents: Methanol and acetonitrile are chromatographically pure (MERCK), and water is di-pure water; the reference substance of rhizoma rhizome (batch number 110794-200303) was purchased from China Institute for the Control of Pharmaceutical and Biological Products; the medicinal materials of Rumex batian were collected in Shanxi and Hebei, my country , Inner Mongolia, Liaoning and other places, it was identified as the dry root of Rumex Patientia L. by Professor Guo Baolin of the Institute of Medicinal Botany, Chinese Academy of Medical Sciences.

[0087] 2 Methods and results

[0088] Determine according to high performance liquid chromatography (Appendix VI D of "Chinese Pharmacopoeia" 2010 edition).

[0089] Chromatographic conditions ...

Embodiment 1

[0101] Example 1 Rumex rhubarb quality standard

[0102] 【Properties】Roots and rhizomes are conical and branched, up to 16cm long and 5cm in diameter. There are stem base remnants and brown-black scales on the root head, and the stem remnants can be in the form of residual brown-black fibers with horizontal stripes underneath. The surface is brownish-gray, with some twisted longitudinal wrinkles and punctate fibrous root scars. Quality tough, difficult to break. The broken surface is irregular, light yellow to brownish yellow, slightly gas, bitter and slightly astringent.

[0103] The decoction pieces of this product are round or irregular thin slices. The cut surface is light yellow to brownish yellow, with annular and radial textures. With fine root marks. Hard, fibrous. Slight gas, bitter and slightly astringent taste.

[0104] [Identification] (1) The cork layer is thin in the cross-section of the root, and there are 2 to 5 bundled or single scattered fibers in the...

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Abstract

The invention relates to a detection method for a traditional Chinese medicine, namely rhubarb root. The detection method is characterized by comprising the following steps of 1. preparing a reference substance solution; 2. preparing a rhubarb root test substance solution; and 3. injecting the reference substance solution and the test substance solution into an ultra performance liquid chromatography so as to obtain a chromatogram map.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicinal materials, in particular to a detection method of rhubarb. Background technique [0002] Rumex is the root of Rumex patientia L., a plant of Polygonaceae. Rumex patientia L. is a perennial herb, its roots are dug in autumn, washed, sliced, sun-dried or used fresh. Roots and rhizomes of rhubarb are conical, brown-red to brown-gray as a whole, with stem base remnants and scale-like and hair-like fibers at the top; branches at the root, with textured lines and long horizontal scars on the surface; hard texture, yellow cross-section, There are brown cambium rings and insertion lines. Slight gas, slightly bitter taste. It is better to have a hard texture and a yellow cross-section. [0003] Tu Dahuang is the monarch drug of Shenyanqing Tablets. The original hospital preparations are only purchased and used locally, and there are no clear regulations on its basic ingredients...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/90
Inventor 周桂荣郭宝林刘艳张兰兰周水平
Owner TIANJIN TASLY PHARMA CO LTD
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