An on-fiber detection system of biomolecules based on photonic crystal fiber

Abstract

The invention belongs to the field of optical fiber sensing technology and discloses an in-fiber detection system for biological molecules based on a pohotonic crystal fiber. The system comprises a light source unit, a sensing unit, a fluid filling unit, an optical detection unit, an optical fiber link and a filling link, wherein light output by a broadband optical source is transmitted to a conical optical fiber through an input optical fiber and an optical fiber circulator. Because the pohotonic crystal fiber is vertically contacted with a conical area of the conical optical fiber, light in the conical area is coupled to enter the pohotonic crystal fiber in a near field coupling manner; light meeting phase matching conditions is limited in the pohotonic crystal fiber to be transmitted; the wavelength corresponding to the light meeting the phase matching conditions is called a resonant wavelength; and light which does not meet the phase matching conditions is re-coupled to enter the conical optical fiber, is reflected into the optical fiber circulator, and then is transmitted to a spectrum analyzer through the output optical fiber so as to serve as a measuring signal. The detection system disclosed by the invention is simple in structure, flexible in design, easy to realize, small in size and high in sensitivity and precision and is capable of performing in-fiber detection on the biological molecules.

Description

technical field [0001] The invention belongs to the technical field of optical fiber sensing and relates to an on-fiber detection system of biomolecules based on a photonic crystal optical fiber. Background technique [0002] At present, the detection of biomolecules, such as nucleic acids and proteins, plays an important role in areas related to national economy and people's livelihood, such as food safety, environmental monitoring, gene analysis and disease diagnosis. In practical applications, rapid, real-time, high-sensitivity and low-cost detection of biomolecules is the goal pursued by many scientific researchers. Traditional biomolecular detection techniques usually include electrochemical methods, fluorescence photometry and isotope methods. Electrochemical method is a relatively mature biochemical detection method, but biomolecules such as protein will interfere with its determination due to adsorption, resulting in low measurement sensitivity. In addition, this ty...

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Problems solved by technology

However, the fabrication of traditional microfluidic channels usually requires complex processes and expensive equipment, which limits the promotion and development of this detection technology.

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