Intelligent tissue engineering blood vessel

Abstract

An intelligent tissue engineered blood vessel. Cardiovascular disease has become a major disease that endangers human health. The most commonly used treatment method is blood vessel transplantation, and a large number of artificial blood vessel substitutes are needed clinically. By intercepting the bilateral carotid arteries of sheep, the method of decellularization and antigen removal by TritonX-100+DNA / RNase digestion was adopted; the slow-release microspheres loaded with VEGF were prepared by emulsification and volatilization method using high molecular substances (PLGA), polyvinyl alcohol and VEGF. ; The decellularized sheep carotid artery stent and slow-release microspheres were tightly combined by freeze-drying to produce tissue-engineered blood vessels loaded with VEGF slow-release microspheres that can directionally induce endothelial cell adsorption, differentiation and proliferation, which is expected to solve the clinical problems encountered in vascular transplantation. Difficulties faced: the limited source of vascular grafts, the rejection of simple heterogeneous vascular grafts, and the poor long-term patency of existing tissue engineered vascular grafts, etc., as a vascular graft substitute for the treatment of human cardiovascular diseases.

Description

technical field [0001] Basic Medicine Clinical Medicine Immunology Tissue Engineering Zoology. Background technique [0002] Cardiovascular disease has become a major disease that endangers human health, and the most commonly used treatment is vascular transplantation. However, due to the limited sources of autologous vascular grafts and the limitations of their own vascular conditions, a large number of artificial vascular substitutes are needed clinically. People have tried various allograft vascular transplantation methods, but their long-term effect is poor, thrombosis, vascular degeneration, hyperplasia, vascular stenosis and occlusion, and some complications occur. Tissue engineering, which has emerged in recent years, provides a new idea for this clinical problem. It uses the basic theories and methods of cell biology, material science, and engineering to decellularize tissues in vitro to remove antigenicity and implant them into the body to replace the host. Tissue...

AI Technical Summary

Problems solved by technology

But up to now, there is no report that small-caliber artificial blood vessel transplantation is fully adapted to clinical practice.

The main reason is that the material has poor hemocompatibility and anticoagulant performance. When it comes into contact with blood, it activates the coagulation system in the body, causing platelets and fibrin to aggregate to form thrombus, resulting in stenosis and occlusion of the lumen.

poor blood compatibility

When it is transplanted into the body, it is easy to cause thrombosis, which seriously affects the recanalization rate of the grafted blood vessels, and is prone to intimal hyperplasia in the long run

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