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Growth accelerant for improving in-vitro culture of hemophilus parasuis

A technology of Haemophilus suis and growth-promoting agent is applied in the direction of adding compounds to stimulate growth, bacteria, microorganisms, etc., which can solve the problems of increasing production costs and processes, and difficulty in culturing Haemophilus parasuis, and achieves wide application value, The effect of reducing the price and increasing the concentration

Active Publication Date: 2017-09-19
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For inactivated vaccines to achieve the ideal immune effect, the antigen concentration is very important. Haemophilus parasuis is difficult to cultivate, and it is generally difficult to meet the requirements for vaccine preparation. Therefore, vaccine manufacturers generally solve this problem by centrifugal concentration. However, this invisibly increases the production cost and process, and brings a lot of inconvenience to the vaccine factory. Therefore, increasing the culture concentration of Haemophilus parasuis is the key to solving this problem, and it can also save a lot of production costs for the vaccine factory. reduce the price of vaccines

Method used

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  • Growth accelerant for improving in-vitro culture of hemophilus parasuis

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The growth-promoting action of embodiment 1 potato juice to Haemophilus parasuis

[0028] Add 5% v / v of carrot juice, tomato juice and potato juice to the conventional culture medium of Haemophilus parasuis respectively, and insert 1% v / v of the culture medium with different vegetable juices into the culture medium of Haemophilus parasuis. Haemobacterium seed solution was cultured under normal conditions, 37°C, 120rpm shaking culture for 24 hours, and counted viable bacteria. At the same time, the original culture medium without any growth-promoting agent was set for cultivation, and the cultivation conditions were the same.

[0029] The above-mentioned existing conventional medium for cultivating Haemophilus parasuis is TSB medium containing NAD and fetal bovine serum, and the specific preparation method is: the purchased TSB (Tryptone Soy Broth) medium is dissolved in water according to the instructions, and dissolved in water at 121 ℃ autoclave for 15 minutes, when ...

Embodiment 2

[0034] The growth-promoting effect of embodiment 2 folic acid to Haemophilus parasuis

[0035] Add folic acid, oxalic acid, and malic acid respectively in the culture medium (same as Example 1) of conventionally cultivating Haemophilus parasuis now, so that its final concentration in the culture medium is respectively 1.5mg / mL, and these 3 kinds of culture 1% v / v seed solution of Haemophilus parasuis was inserted into the medium respectively, cultured under normal conditions, 37° C., 120 rpm shaking culture for 24 hours, and counted viable bacteria. At the same time, the original culture medium without any growth-promoting agent was set for cultivation, and the cultivation conditions were the same.

[0036] The detection results are shown in Table 2, from which it can be seen that when folic acid, oxalic acid, and malic acid are used as growth-promoting agents, the growth-promoting effects on Haemophilus parasuis are different, and oxalic acid and malic acid have different eff...

Embodiment 3

[0040] Embodiment 3 A kind of growth promoting agent that improves Haemophilus parasuis cultured in vitro

[0041] The Haemophilus parasuis growth promoter is prepared according to the following formula, in which the yeast extract needs to be subjected to high-pressure treatment at 121°C, and other components need to be filtered to verify that the growth promoter is sterile. After the growth-promoting agent was prepared, it was stored at 4°C for later use.

[0042] Haemophilus parasuis growth promoter formula:

[0043] Folic acid 5g / mL

[0044] KCl 3mg / mL

[0045] NaH 2 PO 4 4mg / mL

[0046] NaCl 3mg / mL

[0047] Yeast extract 5mg / mL

[0048] NAD 21μg / mL

[0049] Sorbitol 55mg / mL

[0050] Potato juice 4% v / v, balance water.

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Abstract

The invention discloses a growth accelerant for improving in-vitro culture of hemophilus parasuis. The growth accelerant is prepared from folic acid, KCl (potassium chloride), NaH2PO4 (sodium dihydrogen phosphate), NaCl (sodium chloride), yeast extract, NAD (nicotinamide adenine dinucleotide), sorbitol, potato juice and water. The growth accelerant disclosed by the invention has the advantages that the growth concentration of the hemophilus parasuis is obviously improved; a hemophilus parasuis deactivating vaccine with ideal immunity effect can be produced without concentration bacterial liquid, the immunological potency is high, the immunity effect is good, a large amount of production cost is saved for a vaccine plant, the price of the vaccine is reduced, and the wide application value is realized.

Description

technical field [0001] The invention relates to a growth-promoting agent for improving the in vitro culture of Haemophilus parasuis. Background technique [0002] Haemophilus parasuis disease is a new bacterial infectious disease that seriously harms the pig industry in recent years, causing multiple serositis, arthritis and meningoencephalitis in pigs. The incidence rate is generally 10-15%, and the mortality rate It can reach more than 50%, which has brought huge economic losses to the pig industry, and has aroused people's increasing attention. [0003] Because Haemophilus parasuis is highly susceptible to drug resistance, an effective way to control the disease is vaccination. It has been reported in the literature that the use of commercial vaccines or in-house vaccines can control Haemophilus parasuis infection. But there are also examples of failure to prevent with inactivated vaccines. For inactivated vaccines to achieve the ideal immune effect, the antigen concen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12R1/21
CPCC12N1/20C12N1/38
Inventor 贾爱卿王贵平邓胜朝包银莉
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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