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Tissue-culture rapid propagating method for wild bletilla striata

A technology of tissue culture and bletilla striata, which is applied in the field of plant breeding and reproduction, can solve the problems of incomplete embryos, low reproduction rate, difficult seed reproduction, etc., and achieve the effects of promoting strong seedlings to take root, improving soil fertilizer efficiency, and better fertilization effect

Inactive Publication Date: 2017-09-19
河池乐康生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The seeds of Bletilla striata are small and powdery, without endosperm, and incompletely developed embryos. The conditions for seed germination are harsh, difficult to germinate, and difficult to propagate seeds; when using branch propagation, the reproduction rate is low and the reproduction speed is slow, so it is provided A cultivation method that can use Bletilla striata seeds as a provenance, shorten its seedling cultivation time, increase the survival rate of germination and the survival rate of plants after transplanting is imminent

Method used

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  • Tissue-culture rapid propagating method for wild bletilla striata
  • Tissue-culture rapid propagating method for wild bletilla striata
  • Tissue-culture rapid propagating method for wild bletilla striata

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Embodiment 1

[0031] The present embodiment provides a method for rapid propagation of wild bletilla striata tissue culture, specifically comprising the following steps:

[0032] (1) Seed culture stage

[0033] 1) Disinfection of explants: first rinse the Bletilla striata capsules with running water for 26 minutes, then scrub the surface of the capsules with washing powder water, then rinse them twice with distilled water, and then put them on the ultra-clean workbench and disinfect them with 75% alcohol for 2 minutes before using Soak in 3% Xinjiemi for 3 minutes, shake it constantly during the period to make it thoroughly sterilized, then put it on a sterile inoculation tray to air dry naturally, and cut off one end with a sterile scalpel for later use;

[0034]2) Preparation of culture medium: with VW medium as the base medium, add: 55g of potato liquid, 0.4mg of 6-BA, 15g of sucrose, 0.04mg of NAA, 3.5g of agar and 8g of purified water to each liter of substrate. The method is to mix a...

Embodiment 2

[0046] The present embodiment provides a method for rapid propagation of wild bletilla striata tissue culture, specifically comprising the following steps:

[0047] (1) Seed culture stage

[0048] 1) Disinfection of explants: first rinse the Bletilla striata capsules with running water for 29 minutes, then scrub the surface of the capsules with washing powder water, then rinse 3 times with distilled water, then place them on the ultra-clean workbench and disinfect them with 75% alcohol for 3 minutes, then use Soak in 3% Xinjiemi for 4 minutes, shake it constantly during the period to make it thoroughly sterilized, then put it on a sterile inoculation tray to air dry naturally, and cut off one end with a sterile scalpel for later use;

[0049] 2) Preparation of culture medium: take VW medium as the base medium, add: 60g of potato liquid, 0.5mg of 6-BA, 20g of sucrose, 0.05mg of NAA, 3.8g of agar and 10g of purified water to each liter of substrate. The method is to mix and sti...

Embodiment 3

[0061] The present embodiment provides a method for rapid propagation of wild bletilla striata tissue culture, specifically comprising the following steps:

[0062] (1) Seed culture stage

[0063] 1) Disinfection of explants: first wash the Bletilla striata capsules with running water for 32 minutes, then scrub the surface of the capsules with washing powder water, then rinse them with distilled water for 3 times, then place them on a super-clean workbench and disinfect them with 75% alcohol for 3 minutes before using Soak in 3% Xinjiemi for 5 minutes, shake it constantly during the period to make it thoroughly sterilized, then put it on a sterile inoculation tray to dry naturally, and cut off one end with a sterile scalpel for later use;

[0064] 2) Preparation of culture medium: with VW medium as the base medium, add: 65g of potato liquid, 0.6mg of 6-BA, 25g of sucrose, 0.06mg of NAA, 4.0g of agar and 12g of purified water to each liter of substrate. The method is to mix an...

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Abstract

The invention provides a tissue-culture rapid propagating method for wild bletilla striata and relates to the technical field of plant culture propagation. The tissue-culture rapid propagating method for the wild bletilla striata, provided by the invention, comprises the following steps: (1) seed culture stage; (2) plow-land transplanting stage, wherein the seed culture stage comprises the steps of 1) explant disinfection, 2) culture medium preparation, 3) sowing, 4) culture room culture and 5) greenhouse seedling hardening; the plow-land transplanting stage comprises the steps of 1) land preparation, 2) sowing, 3) fertilizer application management and 4) disease and insect pest control. According to the tissue-culture rapid propagating method for the wild bletilla striata, provided by the invention, strict check is carried out from seed culture to plow-land-transplanted cultivation, and all the steps are combined tightly and interact, so that the survival rate of the wild bletilla striata is increased, and the method is applicable to large-scale planting.

Description

【Technical field】 [0001] The invention relates to the technical field of plant cultivation and propagation, in particular to a method for rapid propagation of wild bletilla striata tissue culture. 【Background technique】 [0002] Bletilla striata is a perennial orchid plant with white tuberous stems and large purple flowers. It is mainly used for hemostasis and anti-inflammation, detumescence and granulation, traumatic bleeding, lung tonifying and hemostasis, burns, hemorrhoids, beauty and freckle removal, etc. It has a variety of medicinal and ornamental values. Bletilla striata is an endangered wild orchid medicinal plant. dwindling, [0003] The seeds of Bletilla striata are small and powdery, without endosperm, and incompletely developed embryos. The conditions for seed germination are harsh, difficult to germinate, and difficult to propagate seeds; when using branch propagation, the reproduction rate is low and the reproduction speed is slow, so it is provided A cultiv...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00A01B79/02A01G13/00C05F15/00A01N65/48A01P1/00
CPCA01B79/02A01G13/00A01H4/001A01H4/008A01N65/00A01N65/24A01N65/38A01N65/42A01N65/48C05F3/00C05F3/04C05F5/002C05F9/04C05F11/00C05F17/70Y02E50/30Y02W30/40
Inventor 陈婉霞罗墩唐庆乐
Owner 河池乐康生态农业科技有限公司
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