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Culture method of rieger begonias test tube flowers

A test-tube flower and begonia technology, applied in the biological field, can solve the problem of less roses and begonias, and achieve the effect of small size, simple operation, and rich colors

Inactive Publication Date: 2012-07-25
张曦予 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are very few reports on the flowering of Begonia rosea in test tube at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] From the rose crabapple plants to be tested, select the leaves of rose crabapple that are free from diseases and insect pests and grow vigorously; cut off the young leaves with scissors, wash away the sediment with tap water, add detergent to the water, and then rinse it repeatedly. Soak in 75% alcohol for 50s under sterile conditions, then sterilize in 0.1% mercuric solution for 8 minutes, and finally rinse with sterile water for 6 times, and blot the surface moisture with sterilized filter paper.

[0025]Cut the sterilized leaves into about 1-2cm 2 Gently embed the small squares on the surface of the induction medium, the medium is MS medium, supplemented with 6-BA0.5mg / L, IAA1.5mg / L, and the pH value is 5.8. After 10 days, the cells on the leaf surface gradually expanded, but not When the callus grows, a few small green buds sporadically emerge on the leaf surface on the 19th day, and many cluster buds begin to grow on the 35th day. The culture conditions are tempera...

example 2

[0029] From the rose crabapple plants to be tested, select the leaves of rose crabapple that are free from diseases and insect pests and grow vigorously; cut off the young leaves with scissors, wash away the sediment with tap water, add detergent to the water, and then rinse it repeatedly. Soak in 75% alcohol for 80s under sterile conditions, then sterilize in 0.1% mercuric solution for 15min, and finally rinse with sterile water for 8 times, and blot the surface moisture with sterilized filter paper.

[0030] Cut the sterilized leaves into about 1-2cm 2 Gently embedded in the surface of the induction medium, the medium is MS medium, supplemented with 6-BA1.0mg / L, IAA2.0mg / L, and the pH value is 6.0. After 12 days, the cells on the leaf surface gradually expanded, but not When the callus grows, a few small green buds sporadically emerge on the leaf surface on the 20th day, and many cluster buds begin to grow on the 33rd day. The culture conditions are temperature 21°C, light i...

example 3

[0034] From the rose crabapple plants to be tested, select the leaves of rose crabapple that are free from diseases and insect pests and grow vigorously; cut off the young leaves with scissors, wash away the sediment with tap water, add detergent to the water, and then rinse it repeatedly. Soak in 75% alcohol for 60 seconds under sterile conditions, then sterilize in 0.1% mercuric solution for 10 minutes, and finally rinse with sterile water for 7 times, and blot the surface moisture with sterilized filter paper.

[0035] Cut the sterilized leaves into about 1-2cm 2 Gently embedded in the surface of the induction medium, the medium is MS medium, supplemented with 6-BA0.8mg / L, IAA1.8mg / L, and the pH value is 5.9. At 11 days, the cells on the leaf surface gradually expanded, but not When the callus grows, a few small green buds sporadically emerge on the leaf surface on the 22nd day, and many cluster buds begin to grow on the 36th day. The culture conditions are temperature 21°C...

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Abstract

The invention relates to a culture method of rieger begonias test tube flowers, belonging to the technical fields of plant tissue culture and test tube flowering. The culture method is characterized by taking the mature laminas of the rieger begonias as main explants and successfully culturing the test tube flowers which are small and exquisite, rich in colors and various in shapes and have a unique ornamental value through the approaches such as the disinfection of the explants, the induction of cluster buds, the growth and the propagation of small seedlings, and the flowering culture in thetest tube. The culture method is simple to operate, short in test period and low in production cost and compared with the traditional planting mode, the culture method has the advantages as follows: the produced test tube flowers can be regulated at will in flowering period, escape from the influence of seasons and save energy and areas, thus having important practical significance for beautifying life and work environment, regulating the flowering off period, increasing the new varieties and the new forms of ornamental flowers, exploring the flowering mechanism and the like. Due to the prominent advantages in flower colors, flower postures, flower shapes, flower period and the like, the rieger begonias is very suitable for being used in exploring exquisite test tube flowers so as to improve the ornamental characteristic and create high economic value.

Description

technical field [0001] The invention relates to a method for cultivating test-tube flowers of roses and begonias, which are ornamental flowers, and belong to the field of biotechnology, and specifically belong to the technical field of plant tissue culture and test-tube flower formation. Background technique [0002] Rose Begonia (Begonia mannii), also known as Rieger Begonia, is a perennial evergreen herbaceous flower belonging to the genus Begonia. It is a new species introduced from abroad in recent years. A new strain of Socotra Begonia (Begonia socotrana) hybridization. Its plant shape is very similar to the bulbous begonia, but there is no obvious bulb. Its flowers are beautiful in color, large and colorful, with rose-like appearance, color and fragrance, so it is called rose begonia. It has the following major characteristics: ①The flower color is delicate, soft, gorgeous and colorful (purple, bright red, pink, yellow, orange, white, etc.); ②The flowers are dense and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 张曦予
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