An automatic rehydration decellularization system

Abstract

The invention discloses an automatic fluid-infusing type acellular system and relates to the technical field of a biological material treating device. The invention aims to solve the problems that an acellular device in the prior art cannot adapt to mass production and is complex in fluid-infusing operation. The automatic fluid-infusing type acellular system disclosed by the invention comprises a table concentrator, a cleaning tank, a fluid-infusing device, a liquid level meter and a control unit, wherein the table concentrator comprises a swinging tray; the cleaning tank is arranged on the swinging tray; the cleaning tank comprises a plurality of cleaning tanks; the fluid-infusing device comprises a fluid storage tank, a fluid-infusing pipe and a fluid-infusing pump; one end of the fluid-infusing pipe is communicated with the fluid storage tank while the other end is inserted into the cleaning tanks; the fluid-infusing pump is serially connected with the fluid-infusing pipe; the liquid level meter is used for detecting the liquid level in the cleaning tanks; the control unit is respectively connected with the fluid-infusing pump and the liquid level meter. The automatic fluid-infusing type acellular system can be used for accellular treatment of biological materials.

Description

technical field [0001] The invention relates to the technical field of biological material processing devices, in particular to an automatic fluid replenishment type decellularization system. Background technique [0002] With the development of economy and society, the application of biomaterials in medicine is becoming more and more prominent. Biomaterials refer to materials formed through biological processes, including cells and extracellular matrix. Commonly used biomaterials include bovine pericardium and porcine small intestine. When biomaterials are used clinically, they need to be cleaned, sterilized, and decellularized. [0003] In the prior art, a shaking table is usually used for decellularization. Specifically, a cleaning box is fixed on the tray of the shaking table, biological materials and decellularization reagents are placed in the cleaning box, and then the shaking table is turned on for oscillation. During the process, the inner wall of the cleaning box ...

AI Technical Summary

Problems solved by technology

[0004] However, due to the limitation of the vibration amplitude of the shaking table, the volume of the cleaning box cannot be made very large. If the volume is too large, the distance between the opposite side walls of the cleaning box will be very large. The medium has moved in the opposite direction before it hits the inner wall of the cleaning box, so decellularization cannot be achieved

Therefore, in order to adapt to the oscillation amplitude of the shaker, the decellularization washing box of the prior art usually has a small volume and cannot be adapted to mass production

Moreover, during the process of decellularization, the decellularization reagent will generate foam, and the amount of the reagent will gradually decrease. When there are many biological materials, the amount of reagent added at one time may not be able to completely decellularize the biological material. When the amount is reduced to a certain extent, rehydration is required. The existing technology is to manually add reagents to the cleaning box after the shaker is stopped. The operation is complicated and the efficiency is low, and the amount of added reagents cannot be accurately controlled.

Images