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Preparation method and application of soybean-source zinc chelating peptide and peptide-zinc chelate

A technology of chelating peptides and soybean plants, which is applied in the preparation of zinc peptide chelates, soybean and its protein source zinc chelating peptides, can solve the problems of adverse effects on growth and development, affecting the synthesis and transformation of DNA polymerase, and achieve anti-aging effect

Inactive Publication Date: 2017-09-15
安徽珠峰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, when zinc is deficient, it will affect the synthesis and transformation of DNA polymerase, RNA synthetase and superoxide dismutase in the body, which will adversely affect the growth and development of the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use a high-speed pulverizer to pulverize soybeans, take 500 g of raw materials, add 4 L of 75% (volume percentage) ethanol aqueous solution, stir and soak for 24 h, and change the liquid every 8 h during this period, and filter the precipitate. 4 L of water was washed and then filtered, and after drying, a light brown crude soybean protein isolate was obtained, and the average yield of the crude protein was 69.55%.

Embodiment 2

[0028] Take 4 g of soybean protein isolate, add 120 ml of deionized water, adjust the pH to 7.2 with 1 mol / L NaOH solution, add 0.08 g of papain, and carry out enzymatic hydrolysis reaction on the reaction mixture at 50 ° C, during which every 30 min The pH was adjusted with 1 mol / L NaOH solution to maintain the pH of the reaction system at 7.2, and the hydrolyzate was obtained when the reaction time reached 6 h. The degree of hydrolysis of the hydrolyzate was detected by TNBS method to be 32.39%. When the peptide concentration was 4 mg / ml, its Zn was detected by EDTA titration 2+ The average chelation rate is 87.24%.

Embodiment 3

[0030] Take 5 g of soybean protein isolate, add 120 ml of deionized water, adjust the pH to 2.0 with 1 mol / L HCl solution, add 0.09 g of pepsin, and carry out enzymatic hydrolysis reaction on the reaction mixture at 37 °C to maintain the reaction system at The pH is 1.5. When the reaction time reaches 2 h, put the hydrolyzate in boiling water and inactivate the enzyme for 10 min.

[0031] The obtained hydrolyzate is freeze-dried to obtain crude powder of zinc chelating peptide. The degree of hydrolysis of the hydrolyzate was 13.28% when detected by TNBS method for 2 h. When the peptide concentration was 4 mg / ml, its Zn was detected by EDTA titration 2+ The average chelation rate is 56.02%.

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Abstract

The invention provides a preparation method and application of soybean-source zinc chelating peptide and peptide-zinc chelate. Soybeans as a material are produced into soybean protein isolate by an extraction and separation process, the soybean protein isolate and protease carry out hydrolysis reaction, active peptide with small molecular weight is obtained by enzymolysis, the peptide reacts with zinc salt, and thereby the peptide-zinc chelate is prepared. By adopting the high-quality soybeans as the material, the high-quality soybean protein isolate is prepared by the invention through the extraction and separation process; the protein is put into an enzymatic vessel and added with an appropriate amount of water to prepare protein pulp, the pH value of the soybean protein pulp is regulated, the protease is then added for hydrolysis reaction, the reaction solution is put into boiled water and cooked for enzyme deactivation after hydrolysis is complete, and thereby hydrolysate is obtained; the hydrolysate is centrifuged, and after the supernate is dried, the crude powder of the zinc chelating peptide is obtained. The zinc chelating peptide prepared by the invention can be directly used as a natural antioxidant to delay aging. In addition, the zinc chelating peptide can also be used to prepare an excellent parent for the peptide-zinc chelate, and the peptide-zinc chelator can be chelated with different trace elements to prepare zinc supplements which are used for treating the nutrient element deficiency symptoms of the body.

Description

technical field [0001] The invention belongs to the technical field of deep processing of soybeans and their products, and in particular relates to a preparation method and application of soybeans and their protein source zinc chelating peptides and peptide zinc chelating compounds. Background technique [0002] Metal chelating peptides refer to bioactive peptides that can chelate metal ions. Metal ions such as copper, zinc, iron, and magnesium are widely present in the organs and tissues of the body. When there are excessive metal ions in the body, it is easy to lead to the generation of reactive oxygen species (Reactive oxygen species, ROS), which in turn damages the biomacromolecules in the body, resulting in some degenerative diseases, such as cardiovascular and cerebrovascular diseases, gastrointestinal inflammation and cancer Wait. Metal chelating peptide itself can chelate free metal ions in the body, and play a role in inhibiting or reducing the oxidative damage ca...

Claims

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Application Information

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IPC IPC(8): A23L33/165A23L33/18A23J1/14
CPCA23J1/142A23L33/165A23L33/18A23V2002/00A23V2200/302
Inventor 朱峰赵敏邱玉礼杨东波尹佳君马永乐程伟琪徐淑荣
Owner 安徽珠峰生物科技有限公司
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