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Preparation method of phosphatidyl serine

A technology of phosphatidylserine and serine, which is applied in the direction of edible phospholipid compositions, chemical instruments and methods, compounds of group 5/15 elements of the periodic table, etc., can solve the problem of processing, increased production costs, and low phosphatidylserine content, etc. question

Inactive Publication Date: 2017-09-12
WUHU FOMAN BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the above-mentioned prior art, the purpose of the present invention is to overcome the preparation of phosphatidylserine by enzymatic method or the method of extracting from organisms in the prior art, and in the production process, the content of the prepared phosphatidylserine is not It is particularly high, and often requires post-processing, which will lead to the problem of greatly increasing production costs, thereby providing a simple preparation method and a preparation method for phosphatidylserine with high product purity.

Method used

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Examples

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preparation example Construction

[0016] The present invention provides a kind of preparation method of phosphatidylserine, wherein, described preparation method comprises:

[0017] 1) mixing phosphatidylcholine and chloroform to prepare a mixture M1;

[0018] 2) adding L-serine and water to the mixture M1 to prepare the mixture M2;

[0019] 3) adding phospholipase D to the mixture M2 to obtain a crude product of phosphatidylserine;

[0020] 4) After adding chloroform to the crude product of phosphatidylserine and mixing, collect the chloroform layer;

[0021] 5) Concentrate the chloroform layer collected in step 4), add acetone after concentration, mix with suction and filter, take the filter cake and dry it to obtain phosphatidylserine.

[0022] In the present invention, phosphatidylcholine and chloroform are first mixed, then L-serine and water are added to the mixture, and after mixing, phospholipase D is added to react to obtain the crude product of phosphatidylserine. Basically, after adding chlorofor...

Embodiment 1

[0033] 1) Stir and mix 20g of phosphatidylcholine and 200g of chloroform to prepare mixture M1;

[0034] 2) Add 10 g of L-serine and 300 g of water to the mixture M1, stir and mix to prepare the mixture M2;

[0035] 3) adding 5 g of phospholipase D to the mixture M2 and stirring and mixing for 4 hours at a temperature of 40° C. to obtain a crude phosphatidylserine;

[0036] 4) After adding 300 g of chloroform to the crude product of phosphatidylserine and mixing, let stand for 4 hours, and collect the chloroform layer;

[0037] 5) Concentrate the chloroform layer collected in step 4), add 400g of acetone after concentration, mix with ion exchange resin and perform suction filtration, take the filter cake and vacuum dry it for 12 hours at a temperature of 50°C, then take out the solid , crushed into powder to obtain phosphatidylserine A1. (phosphatidylserine content of 92%)

Embodiment 2

[0039] 1) Stir and mix 20g of phosphatidylcholine and 150g of chloroform to prepare mixture M1;

[0040] 2) Add 8g of L-serine and 260g of water to the mixture M1, stir and mix to prepare the mixture M2;

[0041] 3) adding 3 g of phospholipase D to the mixture M2 and stirring and mixing for 3 hours at a temperature of 38° C. to obtain a crude phosphatidylserine;

[0042] 4) After adding 260 g of chloroform to the crude phosphatidylserine and mixing, let it stand for 1 hour, and collect the chloroform layer;

[0043] 5) Concentrate the chloroform layer collected in step 4). After concentration, add 350 g of acetone to mix, filter through ion exchange resin, and perform suction filtration. Take the filter cake and vacuum dry it for 8 hours at a temperature of 45° C., then take out the solid , crushed into powder to obtain phosphatidylserine A2. (89% phosphatidylserine content)

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Abstract

The invention discloses a preparation method of phosphatidyl serine. The preparation method comprises the following steps of (1) mixing phosphatidylchline and chloroform to prepare a mixture M1; (2) adding L-serine and water into the mixture M1 to prepare a mixture M2; (3) adding phosphatidase D into the mixture M2, thus preparing a phosphatidyl serine crude product; (4) adding chloroform into the phosphatidyl serine coarse product, mixing, and then collecting a chloroform layer; (5) concentrating the chloroform layer collected in the step (4); after the concentration, adding acetone; mixing, then performing suction filtration; taking filter cake; drying to prepare the phosphatidyl serine. The phosphatidylchline and the chloroform are firstly mixed; then, the L-serine and the water are added into the mixture; then, the phosphatidase D is added; the chloroform is added on the basis of the phosphatidyl serine coarse product, and mixing is performed; then the chloroform layer is subjected to concentration, then, concentrated materials are taken and are added into the acetone; the suction filtration is performed; drying is further performed; the high-purity phosphatidyl serine is prepared.

Description

technical field [0001] The invention relates to the field of production and processing of phosphatidylserine, in particular to a preparation method of phosphatidylserine. Background technique [0002] Although the content of phosphatidylserine in the human body is very small, its function is unique. It can affect the transmission of chemical information in the brain and help brain cells store and read data. It is an important nutrient element to maintain the brain's normal memory, response and healthy mood. Moreover, studies have shown that phosphatidylserine has an important regulatory effect on many cell metabolism processes, can improve the vitality of brain cells, and has a good effect on preventing Alzheimer's disease, treating brain atrophy, and improving brain function in the elderly. Reducing the secretion of stress hormones, promoting the recovery of brain fatigue, balancing emotions, and relieving depression all have certain curative effects. [0003] In the prio...

Claims

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Application Information

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IPC IPC(8): C12P13/06C07F9/10
CPCC07F9/10C12P7/6481C12P13/06
Inventor 郑志强
Owner WUHU FOMAN BIOPHARMA CO LTD
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