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New method for breeding wheat with fusarium head blight resistance

A technology of scab and a new method, applied in the field of agricultural science, can solve the problems of high cost of identification, inaccurate identification results, and slow breeding process of scab-resistant wheat, so as to improve work efficiency and accuracy, and reduce screening The effect of workload, large breeding application value

Inactive Publication Date: 2017-09-12
JIANGSU QIANGNONG AGRI TECHNICAL SERVICES CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a method for the slow breeding process of scab-resistant wheat caused by the inaccurate identification results, long identification period and high identification cost of wheat scab resistance identification by optimizing the appropriate concentration. A new method for scab-resistant wheat breeding by treating the florets of hybrid plants with DON toxin to specifically screen wheat breeding lines with high scab resistance and greatly improving the efficiency and accuracy of scab resistance identification

Method used

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  • New method for breeding wheat with fusarium head blight resistance
  • New method for breeding wheat with fusarium head blight resistance
  • New method for breeding wheat with fusarium head blight resistance

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Experimental program
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Embodiment 1

[0055] In 2007, Yannong 19 (resistance to head blight in field) and Lianmai 5 (susceptibility to head blight in field) were used to conduct experiments on different anther development stages of wheat treated with DON toxin, and a gradient concentration of DON toxin was set to treat 5 Experiments were carried out at different anther development stages of wheat, including the unfertilized pollen stage, the first division stage of fertilized eggs, the grain embryo development stage 5 days after fertilization, the "mostly kernel" stage of grains 15 days after fertilization, and the grain filling stage 25 days after fertilization Expect. Due to the different sensitivities to DON toxin at different stages, a series of graded concentrations of DON toxin were set up for tests at different anther development stages of Yannong 19 and Lianmai 5 wheat.

[0056] The specific operation process of treating unfertilized pollen with DON toxin is as follows: dig wheat plants at the heading stag...

Embodiment 2

[0070] From 2009 to 2015, using the technical route of the present invention, Sumai No. 3 was selected as the hybrid male parent, and the resistance to scab of the main breeding parent W0533 was successfully improved, and 4 new scab-resistant wheats were successfully bred. strain.

[0071] (1) Select W0533 and Sumai 3 as hybrid female parent and male parent respectively:

[0072]W0533 is the main parent of wheat breeding used by our unit for many years. It has many advantages such as high yield, excellent rice quality, and lodging resistance. The ear width should be significantly larger than the sowing width; the wheat feet are tightened, and the space is in a typical inverted "V" shape. The base of the plant shows good permeability, but W0533 has poor resistance to scab. The identification of resistance to scab-affected areas for many years showed that the diseased ear rate of W0533 was 35% to 65%, and the yield was generally reduced by 8% to 20%. Sumai No. 3 was bred in 19...

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Abstract

The invention discloses a new method for breeding wheat with fusarium head blight resistance. The method comprises the following steps: (1) selecting a wheat variety / line to be improved and a fusarium head blight-resistant source, and carrying out hybridization to obtain F1; (2) using DON toxin of a gradient concentration to treat plant florets of male and female parents at a heading period, and determining fusarium head blight resistance screening pressure; (3) backcrossing the cross-breed F1 and the female parent to obtain BCF1; (4) treating plant florets of the BCF1 by using the fusarium head blight resistance screening pressure to obtain BCF2; (5) treating plant florets of the BCF2 by using the fusarium head blight resistance screening pressure again to obtain BCF3; (6) screening agronomic traits of the BCF3, and carrying out advanced generation selfing screening so as to obtain an improved new wheat line with the fusarium head blight resistance. According to the method, the DON toxin of the proper concentration is preferentially selected to treat the plant florets of the cross-breeds, so that a wheat breeding family with high fusarium head blight resistance can be specifically screened out; the method greatly increases the working efficiency and accuracy of fusarium head blight resistance identification, and has a higher application value in the aspect of breeding.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method of treating the florets of hybrid plants with DON toxin at an optimal concentration to specifically screen wheat breeding families with high resistance to scab and greatly improve the identification of scab resistance Novel method for head blight resistance wheat breeding with efficiency and accuracy. Background technique [0002] Wheat head blight (Fusarium head blight, FHB), also known as red head blight, rotten wheat head, wheat ear blight, red wheat head, wheat Fusarium head blight, is a worldwide disease caused by Fusarium fungus. One of the fungal diseases that threatens production the most. Wheat head blight mainly occurs in areas with warm and humid climates in East Asia and South America, but in the past decade, due to global warming and changes in farming systems, it has also occurred frequently in North America and Europe. Wheat scab in my ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 李晶瑶
Owner JIANGSU QIANGNONG AGRI TECHNICAL SERVICES CO LTD
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