Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for extracting levodopa from conversion solution

A technology of levodopa and conversion solution, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as difficult wastewater treatment, harm to health, environmental pollution, etc., and reduce processing pressure and reduce processing pressure. cost, cost reduction effect

Inactive Publication Date: 2017-09-08
SHANDONG LUKANG PHARMA
View PDF5 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Microbial enzyme conversion method is a kind of mild reaction conditions, environmentally friendly preparation method of levodopa, however, at present, there are few reports on the industrialization of levodopa preparation by microbial enzyme method, mainly because the conversion liquid purification method produces Wastewater is more difficult to treat
Chinese patents CN 106117071A and CN106117072A have announced two methods for purifying levodopa in the conversion liquid, but these two methods do not provide a treatment method for purifying waste water, and the mother liquor waste water or centrifugal waste water of these two extraction processes all contain Catechol, the cost of follow-up treatment is higher
Phenol-containing wastewater is harmful to health and causes serious environmental pollution. At the same time, levodopa in wastewater cannot be recovered
Therefore, the high concentration of catechol in the current wastewater limits the industrialization of levodopa microbial enzymatic conversion method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The extraction process of embodiment 1 levodopa transformation liquid

[0051] a. Escherichia coli genetically engineered bacteria are E.coli M15, purchased from Hong Kong Biaoxin Special Co., Ltd. After the transformation reaction is completed, the volume of the transformation solution is 500L, the content of levodopa is 48g / L, and the content of catechol is 4g / L, add 1Kg ascorbic acid to the transformation solution to prevent levodopa and catechol from being oxidized in the subsequent treatment process;

[0052] b. Flow hydrochloric acid into the conversion solution, adjust the pH to 1.0, and stir until the levodopa particles precipitated by the reaction are completely dissolved and continue to stir for 30 min;

[0053] c. After the stirring, centrifuge the transformation solution with a high-speed centrifuge, control the rotating speed to 4000rpm, the temperature is 20°C, and the time is 30min, centrifuge to remove some insoluble impurities such as insoluble bacteria...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting levodopa from a conversion solution. The method comprises the steps of adding inorganic acid into the conversion solution in a flow manner, then, carrying out adsorption with absorbent resin, carrying out eluting, and the like. According to the method, the existing process of firstly carrying out levodopa extraction and then treating pyrocatechin-containing wastewater is abandoned, an extraction process for recovering pyrocatechin while carrying out levodopa extraction is invented, subsequent wastewater treating pressure is alleviated, and unexpended pyrocatechin is recovered while the levodopa is purified, so that subsequent wastewater treatment becomes simple, the extraction cost is greatly reduced, and the subsequent sewage treatment cost is reduced.

Description

technical field [0001] The invention belongs to the field of post-fermentation extraction, and in particular relates to a method for extracting levodopa from transformation liquid. Background technique [0002] Levodopa, also known as 3,4-dihydroxyphenylalanine, is a white crystalline powder, odorless and tasteless, insoluble in ethanol, chloroform and acetone, soluble in dilute acid, as an important biologically active substance , Levodopa is the precursor of the neurotransmitter dopamine, which can pass through the blood-brain barrier, enter the cerebral circulation, and reach the central nervous system. Treat Parkinson's disease. [0003] Levodopa is the first choice and the most effective drug for the treatment of senile Parkinson's disease. In addition, Levodopa also has the effect of treating amblyopia and heart failure. Although there will be some side effects after long-term use, more than 40 years old Years have passed, and there are still no better alternatives. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C229/36C07C227/40C07C227/42C07C39/08C07C37/72C07C37/84C12P13/04
CPCC07C227/40C07C37/009C07C37/685C07C37/72C07C37/84C07C227/42C12P13/04C07C229/36C07C39/08
Inventor 张兴灿朱维忠郑长春王博金伟孙明纯张勇
Owner SHANDONG LUKANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products