Method for extracting levodopa from conversion solution
A technology of levodopa and conversion solution, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as difficult wastewater treatment, harm to health, environmental pollution, etc., and reduce processing pressure and reduce processing pressure. cost, cost reduction effect
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Embodiment 1
[0050] The extraction process of embodiment 1 levodopa transformation liquid
[0051] a. Escherichia coli genetically engineered bacteria are E.coli M15, purchased from Hong Kong Biaoxin Special Co., Ltd. After the transformation reaction is completed, the volume of the transformation solution is 500L, the content of levodopa is 48g / L, and the content of catechol is 4g / L, add 1Kg ascorbic acid to the transformation solution to prevent levodopa and catechol from being oxidized in the subsequent treatment process;
[0052] b. Flow hydrochloric acid into the conversion solution, adjust the pH to 1.0, and stir until the levodopa particles precipitated by the reaction are completely dissolved and continue to stir for 30 min;
[0053] c. After the stirring, centrifuge the transformation solution with a high-speed centrifuge, control the rotating speed to 4000rpm, the temperature is 20°C, and the time is 30min, centrifuge to remove some insoluble impurities such as insoluble bacteria...
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