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Application of acanthopanax sessiliflorus fruit polyphenol

A short-stemmed Acanthopanax, product technology, applied in medical preparations containing active ingredients, food science, plant raw materials, etc., can solve the problem of unclear mechanism of action of anti-CAM, and achieve the effect of inhibiting the expression of cell adhesion molecules

Inactive Publication Date: 2017-09-08
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing drugs such as statins, antiplatelet, anti-inflammatory or immunosuppressive drugs, antioxidants and some compounds in natural plants can inhibit the expression of CAMs caused by inflammation, but the mechanism of action of anti-CAM is still unclear

Method used

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  • Application of acanthopanax sessiliflorus fruit polyphenol
  • Application of acanthopanax sessiliflorus fruit polyphenol
  • Application of acanthopanax sessiliflorus fruit polyphenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1, Extraction and Purification of Polyphenols from Acanthopanax Acanthopanax

[0050] 1. Extraction and content determination of polyphenols

[0051] Weigh 100g of dried Acanthopanax punctatus powder and extract with 10 times and 8 times of 50% ethanol (volume fraction) at 80°C for reflux twice, each time for 1 hour, combine the extracts, filter them with suction, and recover the ethanol from the filtrate under reduced pressure Freeze-drying to obtain the crude polyphenols of Acanthopanax acanthus.

[0052] Aluminum nitrate complex method was used to determine the content of polyphenols in Acanthopanax acanthus, the specific steps are as follows:

[0053] (1) Preparation and detection of reference substance solution:

[0054] Accurately weigh about 5.0 mg of chlorogenic acid standard substance, put it in a small beaker, add appropriate amount of distilled water to ultrasonically dissolve, add distilled water to make up to 25mL, and use it as a reference soluti...

Embodiment 2

[0101] Example 2, the effect of polyphenols of Acanthopanax polyphenols on TNF-α-induced adhesion molecules in human umbilical vein endothelial cells

[0102] Human Umbilical Vein Endothelial Cells (HUVEC)

[0103] 1. Culture of primary human umbilical vein endothelial cells

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Abstract

The invention provides application of acanthopanax sessiliflorus fruit polyphenol. The preparation of acanthopanax sessiliflorus fruit polyphenol includes: respectively using 10 times of and 8 times of 50% ethanol for reflux extraction, combining the extracted liquid, conducting pumping filtration, subjecting the filtrate to pressure reduced recovery of ethanol, and then performing freeze-drying to obtain an acanthopanax sessiliflorus fruit crude polyphenol product; employing a D101 macroporous resin column to purify the acanthopanax sessiliflorus fruit crude polyphenol product, then using distilled water, 30% (volume fraction), 50%, 70% and 95% ethanol for elution in order, collecting eluent generated by 50% ethanol elution, recovering ethanol, conducting pressure reduced concentration, and then performing freeze-drying, thus obtaining canthopanax sessiliflorus fruit polyphenol (ASFP). Application of the acanthopanax sessiliflorus fruit polyphenol in preparation of products for preventing and / or treating the expression of cell adhesion molecules also belongs to the protection range of the invention. The acanthopanax sessiliflorus fruit polyphenol is extracted from mature acanthopanax sessiliflorus fruits, has the effects of preventing and / or treating the expression of cell adhesion molecules, and can be widely applied to the food, health food, and medical and health fields.

Description

technical field [0001] The present invention relates to the application of polyphenols of Acanthopanax acanthus. Background technique [0002] Cell adhesion molecules (CAM) are transmembrane proteins that mediate the adhesion and interaction between cells and cells or extracellular matrix. In pathological conditions such as hyperlipidemia, hypertension and diabetes, CAM stimulates the recruitment of leukocytes Access to the vascular endothelium is an important step in the pathogenesis of atherosclerosis. There are currently no drugs that selectively inhibit the expression and activation of CAMs on the market. Existing drugs such as statins, antiplatelet, anti-inflammatory or immunosuppressive drugs, antioxidants and some compounds in natural plants can inhibit the expression of CAMs caused by inflammation, but the mechanism of action of anti-CAM is still unclear. Acanthopanax acanthus contains caffeic acid, hyperin, quercetin gallic acid, p-hydroxyphenylethyl alcohol and o...

Claims

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Application Information

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IPC IPC(8): A61K36/254A61P29/00A61P9/10A23L33/105A23L2/38A61K131/00
CPCA23L2/38A23L33/105A61K36/254A23V2002/00A61K2236/55A61K2236/39A61K2236/333A61K2236/15A23V2200/30A23V2250/2132
Inventor 李盛钰高磊段翠翠赵玉娟牛春华栾畅赵文博
Owner JILIN ACAD OF AGRI SCI
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