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Method for detecting CNV (Copy Number Variation) mark of a gene GBP2 of Qinchuan cattle and application of CNV mark

A technique of GBP2-CNV1 and GBP2-CNV2, which is applied in the field of detection of GBP2 gene copy number variation of Qinchuan cattle, achieves the effect of accurate and reliable method and easy operation

Inactive Publication Date: 2017-09-01
NORTHWEST A & F UNIV
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Problems solved by technology

Some studies have been done on the function of the GBP2 gene in humans and mice, and found that it has an important impact on the regulation of cell growth and disease resistance, but there are few reports on cattle

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  • Method for detecting CNV (Copy Number Variation) mark of a gene GBP2 of Qinchuan cattle and application of CNV mark
  • Method for detecting CNV (Copy Number Variation) mark of a gene GBP2 of Qinchuan cattle and application of CNV mark
  • Method for detecting CNV (Copy Number Variation) mark of a gene GBP2 of Qinchuan cattle and application of CNV mark

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Embodiment Construction

[0035] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0036] The present invention uses real-time fluorescent quantitative PCR to detect the copy number variation of Qinchuan cattle GBP2 gene and is used for molecular breeding, usually including the following steps:

[0037] (1) Use the NCBI database to find the GBP2 gene sequence, then use the Primer5 software to design primers, and use ordinary PCR to detect the primers;

[0038] (2) Using real-time fluorescent quantitative PCR (qPCR) technology to detect the copy number variation of the candidate loci in the population, and to screen for CNV markers related to the growth traits of Qinchuan cattle;

[0039] (3) Use SPSS 20.0 software to conduct correlation analysis between the type of copy number variation and the growth traits of Qinchuan cattle;

[0040] (4) Breeding Qinchuan cattle with excellent growth traits according to the type of copy number var...

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Abstract

The invention discloses a method for detecting a CNV (Copy Number Variation) mark of a gene GBP2 of Qinchuan cattle and the application of the CNV mark. The method comprises the following steps: on the basis of a real-time fluorescent quantitation PCR (Polymerase Chain Reaction) technology, by taking the DNA of a whole genome of blood of the Qinchuan cattle as a template, respectively multiplying two CNV regions of the gene GBP2 of the cattle by using two pairs of specific primers GBP2-CNV1 and GBP2-CNV2, multiplying a gene BTF3, which serves as a contrast, of the Qinchuan cattle by using another pair of specific PCR primers R1, and judging whether the copy number of individuals is missing or not according to a calculation result of 2-delta delta Ct. According to the method provided by the invention, on the basis of the relevance between missing of the copy number of the gene GBP2 of the Qinchuan cattle and a growth character, the Qinchuan cattle molecular mark assistant selection breeding work is accelerated favorably. The method is simple, quick and convenient for popularization and application.

Description

technical field [0001] The invention belongs to the field of molecular genetics research, and specifically relates to a method for detecting the variation in the copy number of the GBP2 gene of Qinchuan cattle. -ΔΔCt Values ​​to determine individual copy number variations. Background technique [0002] With the rapid development of related disciplines such as genomics and bioinformatics, major changes have taken place in the theory and technology of animal genetics and breeding, and the breeding of beef cattle has also shifted from traditional conventional phenotype selection to molecular selection. At present, molecular breeding His research mainly focuses on marker-assisted selection (MAS), which is a technique to select genetic resources or breeding materials through DNA molecular markers, and to improve the comprehensive traits of livestock and poultry. In livestock and poultry breeding, through the selection of DNA markers that are closely related to growth traits and ...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 黄永震张桂民宋成创贺花陈宏雷初朝邓立蓝贤勇党瑞华白跃宇胡沈荣
Owner NORTHWEST A & F UNIV
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