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Application of gibberella intermedia fermentation extract to prevention and control of plant inset pests

A technology of plant pests and extracts, applied in the fields of application, plant growth regulators, botanical equipment and methods, etc., can solve the problem of affecting tomato yield and quality, aggravating fusarium wilt, root rot, soil-borne fungal diseases and some bacterial diseases, Poor environmental compatibility and other issues, to achieve good prevention and control effect, short cycle, easy to degrade effect

Inactive Publication Date: 2017-08-29
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, root-knot nematode damage also aggravates the occurrence of soil-borne fungal diseases such as fusarium wilt and root rot and some bacterial diseases, seriously affecting the yield and quality of tomatoes
Similar to the control of diamondback moth, tomato root-knot nematode disease also mainly relies on chemically synthesized pesticides, which also bring problems such as poor environmental compatibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Gibberella intermedia Preparation of QJP2 Fermented Extract

[0012] (1) Fermentation culture

[0013] According to the conventional culture method of microorganisms, pick a small amount of strains preserved on the slant of PDA medium Gibberella intermedia QJP2 was inoculated on the surface of a PDA plate, cultured at 28°C for 5 days, and used as a strain for large-scale fermentation culture.

[0014] PDA medium: 200 g of potatoes, 20 g of glucose, 12 g of agar, 1000 mL of distilled water, and adjust the pH to 7.0.

[0015] The strains on the surface of the above PDA plate were cut, inoculated into a sterilized Erlenmeyer flask filled with MH2 medium, and cultured on a shaker at 28°C and 120 rpm for 10 days.

[0016] MH2 Medium: Mannitol 2%, Glucose 2%, KH 2 PO 4 0.05%, MgSO 4 • 7H 2 O 0.03%, yeast extract powder 0.3%, corn steep liquor 0.1%, artificial seawater (NaCl 3%), pH 6.5.

[0017] (2) Preparation of fermented extract

[0018] Filter the ...

Embodiment 2

[0019] Embodiment 2: Plutella xylostella insecticidal activity assay

[0020] The insecticidal activity of the fermented extracts of the strains on the 2nd instar larvae of Plutella xylostella was determined by the leaf disc dipping method.

[0021] Operation steps: (1) Cut fresh rapeseed leaves into 1 cm × 1 cm leaflet discs, and prepare the sample (fermented extract prepared in Example 1) with 90% acetone solution to a certain concentration; (2) Pipette 50 μL of the sample solution into a 24-well plate equipped with 4 leaf discs, 8 replicates per concentration, and use 90% acetone solution as a blank control; (3) After the solvent evaporates, add the 2nd instar larvae of diamondback moth, Well 1 head, covered with a lid, and cultured in the dark at 35°C and RH40%; (4) After 72 hours of culture, observe the feeding and death of diamondback moth larvae, and calculate the corrected mortality rate. Corrected mortality rate = (A-CK) / (1-CK)×100%, A is the lethality rate of the sa...

Embodiment 3

[0023] Example 3: Nematicidal Activity

[0024] (1) Experimental method

[0025] Cultivation method of test nematodes

[0026] Weigh 20 g of oatmeal and add 60 mL of water, add 1 mL of tomato root-knot nematode suspension after sterilization, and incubate at 25°C for 7 days. Under sterile conditions, pick an appropriate amount of cultured nematode-containing oats and put them on the filter paper, wash out the nematodes with a simple Berman funnel method, and suspend the nematodes in sterile water for later use, the concentration is about 2000 / mL, 4 Store at ℃ for later use.

[0027] Nematicidal Activity Assay

[0028] Take a certain amount of sample (fermentation extract prepared in Example 1), dilute 50 and 100 times with 50% DMSO aqueous solution, draw 1 mL into a sterilized centrifuge tube, add 100 μL of nematode suspension, and dilute with 50% DMSO DMSO treatment was used as the control, and it was left standing at 25°C for 12h. After mixing, pipette the mixed solutio...

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PUM

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Abstract

The invention discloses application of gibberella intermedia QJP2 fermentation extract to prevention and control of plant inset pests and pathogenic nematodes and belongs to the field of microbial pesticide. A strain is preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number is CGMCC No. 13199. The gibberella intermedia QJP2 fermentation extract is obtained by fermenting the strain through a shaking table and extracting with ethyl acetate and has relatively insecticidal activity on the plant inset pests including Plutela xylostella and the like; the fatality rate reaches 85.6+ / -1.3 percent when the concentration of the gibberella intermedia QJP2 fermentation extract is 1.0mg / mL; moreover, the gibberella intermedia QJP2 fermentation extract also has relatively good inhibition activity on the root knot nematode disease of tomatoes, and the fatality rates of 50-time and 100-time diluents of the gibberella intermedia QJP2 fermentation extract on the root knot nematode disease of the tomatoes reach 95 percent and 81 percent respectively. Therefore, the gibberella intermedia QJP2 fermentation extract can be used for preparing the microbial pesticide for preventing and controlling the plant inset pests and the pathogenic nematodes; compared with chemically synthetized pesticide, the microbial pesticide also has the advantages of environmental friendliness, low toxin to human and livestock and the like and has a relatively good application prospect.

Description

technical field [0001] The invention belongs to the field of biological pesticides, specifically Gibberella intermedia Application of QJP2 fermented extract in controlling plant pests and pathogenic nematodes. Background technique [0002] diamondback moth Plutela xylostella L. (DBM) belongs to the family Lepidoptera and is an important pest of Brassicaceae plants. Plutella xylostella has many generations and overlaps seriously, has strong adaptability to the environment, and has a high reproductive coefficient. It is distributed in various provinces and regions in my country. Seriously damage cabbage, cauliflower, kohlrabi, mustard greens, cabbage, radish, and rapeseed, and also eat potatoes, tomatoes, green onions, and onions, and even include ornamental plants such as violets, osmanthus, and medicinal plants such as Radix. For many years, the control of diamondback moth has been mainly using chemically synthesized pesticides, which not only increases the resistance o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P7/04A01P5/00
CPCA01N63/10
Inventor 杜丰玉肖琳金静周远明居广琳
Owner QINGDAO AGRI UNIV
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