Bactericidal composition
The technology of a composition and isobacteron is applied in the field of pesticides to achieve the effects of reducing the amount and frequency of use of pesticides, reducing production and use costs, and delaying the drug resistance of pathogenic bacteria
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Embodiment 1
[0016] Biological Assay Example 1: Indoor Toxicity Determination Test of Mandestrobin and Iprodione Compound to Strawberry Botrytis Botrytis
[0017] Test object: Wild-type sensitive Botrytis cinerea strain (Botrytis cinerea Pers)
[0018] experiment method:
[0019] Determination by leaf inhibition method (refer to NY / T 1156.9-2008). On the basis of the pre-test, mandestrobin and iprodione were prepared to the required concentration, ready to use; from the test strawberry, cut healthy leaves with 1cm-2cm petioles that were fully expanded, uniform in leaf age, and wiped with wet cotton. The petiole is wrapped by the ball and placed in a petri dish, and kept moist for later use; the liquid medicine is evenly sprayed on the back of the leaves, and 10 leaves are treated, and repeated 4 times. After the medicinal solution was naturally air-dried, put the leaves of each treatment with their backs up, and discharge them in a petri dish according to the treatment label to keep them...
Embodiment 2
[0030] Bioassay Example 2: Indoor virulence assay test of mandestrobin and iprodione compound against Sclerotinia sclerotiorum
[0031] Test object: Sclerotinia sclerotiorum (Lib.) de Bary)
[0032] experiment method:
[0033] Indoor growth rate method was used (Zhou Feng, Synergistic effect of tebuconazole and sclerotinia on the pathogenic bacteria of Sclerotinia sclerotiorum, 2013, 52(8): 602-605). On the basis of the preliminary test, mandestrobin and iprodione were prepared into PDA medium plates with different concentrations, and a fresh sclerotia cake with a diameter of 6mm was placed in the center of each plate, and each treatment was repeated 4 times. , cultivated in an incubator at 26°C for 48 hours, measured the colony diameter by the cross method, and took the average value, in mm.
[0034] The CTC (Co-toxicity Coefficient) adopts Sun Yunpei's method, specifically referring to the method described in Example 1 of biological assay.
[0035] Table 2 Indoor toxicity...
Embodiment 1
[0041] 51% mandestrobin. Iprodione water dispersible granules
[0042]
[0043] The mandestrobin original drug, iprodione original drug and corresponding auxiliary agents are weighed according to the mass ratio, and the water-dispersible granules of the present invention are prepared after crushing, mixing, granulating and sieving by wet granulation.
[0044] In this embodiment, the mass ratio of mandestrobin to iprodione can be changed between 50:1-1:50, and the total mass percentage of the two is still 51%, forming a new embodiment.
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