Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue culture method of tissue culture seedling of moringa oleifera

A technology for tissue culture and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of yellowing and easy breaking of seedlings, and achieve the goal of improving the degree of lignification, shortening the cycle, and saving costs Effect

Inactive Publication Date: 2017-08-29
上海杉一植物科技有限公司
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] This experiment aims at the subculture process of a kind of Moringa oleifera, and solves the problem of yellowing and fragility of the tissue culture seedlings in the rapid propagation of Moringa oleifera

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture method of tissue culture seedling of moringa oleifera
  • Tissue culture method of tissue culture seedling of moringa oleifera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A tissue culture rapid propagation method of Moringa, comprising the following steps:

[0037] (1) Experimental material must be selected: the Moringa oleifera tissue culture seedlings whose growth is neat, plant height is 3.5-4.5cm, subculture cycle is 21-25 days, leaf color is green or tender green are selected as culture material;

[0038] (2) Specific experimental steps:

[0039] (2.1) Cutting of the material: remove the leaves, keep the top buds and heart leaves, leave the petiole 0.1-0.2 cm, cut into 0.5-0.8 cm stem segments, each segment with 1-2 axillary buds;

[0040] (2.2) subculture of Moringa oleifera; terminal buds and stem sections are inoculated separately, and evenly inoculated into No. 8 subculture medium (LM-8);

[0041] Among them, the improved MS (KNO 3 0.95-1.43g / L, NH 4 NO 3 0.81-1.24g / L, MgSO 4 ·7H 2 O 0.19-0.28g / L, KH 2 PO 4 0.09-0.13g / L, CaCl 2 2H 2 O 0.22-0.44g / L, Na 2 EDTA·2H 2 O0.002-0.04g / L, FeSO 4 ·7H 2 O 0.015-0.03g / L, MnSO ...

Embodiment 2

[0048] A tissue culture rapid propagation method of Moringa, comprising the following steps:

[0049] (1) Experimental material must be selected: the Moringa oleifera tissue culture seedlings whose growth is neat, plant height is 3.5-4.5cm, subculture cycle is 21-25 days, leaf color is green or tender green are selected as culture material;

[0050] (2) Specific experimental steps:

[0051] (2.1) Cutting of the material: remove the leaves, keep the top buds and heart leaves, leave the petiole 0.1-0.2 cm, cut into 0.5-0.8 cm stem segments, each segment with 1-2 axillary buds;

[0052] (2.2) subculture of Moringa oleifera; terminal buds and stem sections are inoculated separately, and evenly inoculated into No. 8 subculture medium (LM-8);

[0053] Among them, step (2.2) improved MS (KNO 3 0.95-1.43g / L, NH 4 NO 3 0.81-1.24g / L, MgSO 4 ·7H 2 O 0.19-0.28g / L, KH 2 PO 4 0.09-0.13g / L, CaCl 2 2H 2 O 0.22-0.44g / L, Na 2 EDTA·2H 2 O 0.002-0.04g / L, FeSO 4 ·7H 2 O 0.015-0.03g...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Root lengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a tissue culture method of a tissue culture seedling of moringa oleifera. The tissue culture method comprises the following steps of (1) selecting experiment materials; (2) specific experiment steps: (2.1) obtaining of a sterile explant: cutting leaves, remaining a terminal bud and interior leaves, remaining a 0.1 to 0.2cm petiole, cutting into 0.5 to 0.8cm stem sections, and remaining 1 to 2 axillarybuds on each section; (2.3) subculture of the moringa oleifera; (2.4) culturing for 21 to 25 days, wherein the height of the longitudinal growth plant is 4.5cm or higher, the side buds of the plant respectively germinate, and the statistic average proliferation ratio reaches 1:6; (2.5) culturing for 18 days in an LM-8 culture medium, wherein the average height of the plant reaches 4cm, the rooting rate reaches 98%, the root length is greater than 2cm, and the number of roots is greater than 3; (2.6) domesticating of the tissue culture seedling: transplanting the rooting tissue culture seedling of the moringa oleifera into a greenhouse, unscrewing a bottle cap, exercising the seedling for 2 to 3 days, and transplanting to a sand bed, wherein the survival rate reaches 95% or above. The tissue culture method has the advantages that the problems of yellowing, crispness and easiness in breaking in the tissue culture process are solved, the expanding propagation and rooting are completed in the bottle at one step, and the cost is reduced by 40% or more.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a tissue culture method of Moringa oleifera tissue culture seedlings, and belongs to the field of biotechnology. Background technique [0002] Moringa (Moringa oleifera Lam.), also known as drumstick tree, milk tree, Chinese toon tree, horse radish, and bean, is a deciduous woody vegetable and oil plant of the genus Moringaceae (Moringaceae) The plant is named Moringa because of its spicy root. Moringa is native to the southern foothills of the Himalayas in northern India, and is now widely planted in more than 30 tropical and subtropical countries and regions in Asia, Africa and America. [0003] The whole body of Moringa is a treasure, and its roots, stems, leaves, flowers, fruits, seeds, etc. can be used, and are rich in nutrients. It is widely used in various fields such as agriculture, industry, animal husbandry, medicine, and beauty. A rare dish integrating delicacy, nutrition a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 李文剑
Owner 上海杉一植物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com