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A carboplatin-resistant ovarian cancer cell line and its application

A technology for ovarian cancer cells and ovarian cancer, applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc.

Active Publication Date: 2020-09-29
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no carboplatin-resistant OVCAR-3 cell line

Method used

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  • A carboplatin-resistant ovarian cancer cell line and its application
  • A carboplatin-resistant ovarian cancer cell line and its application
  • A carboplatin-resistant ovarian cancer cell line and its application

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Experimental program
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Effect test

Embodiment 1

[0015] 1. Materials

[0016] OVCAR-3 cells were purchased from ATCC, USA.

[0017] Carboplatin (CBP) injection was purchased from Qilu Pharmaceutical Co., Ltd.: 50mg / 10ml / support.

[0018] Carboplatin working solution was prepared with cell culture medium (RPMI-1640+10% FBS, purchased from Gibco, USA).

[0019] 2. Steps of building a plant

[0020] 1) OVCAR-3 cells were cultured with RPMI-1640+10% FBS+100 U / ml penicillin+0.1 mg / ml streptomycin.

[0021] 2) OVCAR-3 cells were intervened with CBP at an initial concentration of 30 μM, and the drug-free medium (RPMI-1640+10% fetal bovine serum) was changed after 24 hours of CBP administration.

[0022] 3) Because carboplatin acts on DNA, the cells do not die immediately after adding the drug, and it takes about a month for the cells to return to a normal state after adding the drug once. After the cells grow stably, the shape is plump, and there are no floating dead cells, then start normal passage (P). After adding 30 μM car...

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Abstract

The invention relates to a carboplatin-resistant ovarian cancer cell line and an application thereof. According to the invention, the carboplatin-resistant ovarian cancer cell line is established in the manner of determining the drug-induced intervention time by carefully observing the cell growth form; the preservation number is CCTCC NO: C201736; the drug resistance of the carboplatin-resistant ovarian cancer cell line is obviously higher than that of the other cell lines which are established in the same period; the carboplatin-resistant ovarian cancer cell line shows ultra-stable drug resistance and can be used as a reliable biological model for researching the happening and development mechanism of ovarian cancer and for finding a therapeutic method for the ovarian cancer after resisting against the drug.

Description

technical field [0001] The present invention relates to the establishment of drug-resistant cell lines, in particular to a carboplatin (carboplatin, CBP) ovarian cancer cell line and its application. Background technique [0002] Ovarian cancer is the first reproductive tract malignant tumor that causes female death. Clinically, the main treatment method is platinum-based chemotherapy combined with paclitaxel on the basis of tumor cytoreductive surgery. The initial treatment effect of chemotherapy is good, but drug resistance is prone to occur. The treatment effect after drug resistance is poor. Common ovarian cancer cell lines have limitations in studying the mechanism of drug resistance in ovarian cancer, and carboplatin-resistant cell lines will be more able to simulate the occurrence of drug resistance in clinical ovarian cancer. [0003] Chinese patent document ZL201410708515.7, authorized announcement date: January 25, 2017, discloses a paclitaxel-resistant ovarian ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12Q1/02C12R1/91
CPCC12N5/0693C12N2503/02G01N33/5011G01N33/5044
Inventor 许国雄张劲国张凌云
Owner JINSHAN HOSPITAL FUDAN UNIV
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