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Yak bone protein peptide with ACE inhibiting function and preparation method and application thereof

A technology of yak bone and protein peptides, which is applied in the field of yak bone protein peptides and its preparation, can solve problems affecting product quality and unclear main components of protein peptides, etc.

Active Publication Date: 2017-08-22
ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in these preparation methods, the pH is usually adjusted by adding acid and alkali, which affects the quality of the product. At the same time, the main composition of the protein peptide is not clear.
my country is rich in yak bone resources, and there is no patent literature report on the use of yak bone protein resources to prepare angiotensin-converting enzyme inhibitory peptides in domestic and foreign patents

Method used

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  • Yak bone protein peptide with ACE inhibiting function and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Preparation method of yak bone protein peptide with ACE inhibitory function

[0067] (1) Choose 100 grams of frozen fresh yak bones, wash them with clean water that meets the drinking water hygiene standards, and use a ox bone crusher to beat the yak bones into bone particles after cleaning the yak bones, add water twice the total amount of yak bone particles in the Treat at 125° C. for 4 hours, obtain yak bone cooking liquid without bone slag by centrifugal filtration, cool to 20° C., and remove fat through a liquid separation tank to obtain yak bone protein liquid.

[0068] (2) Adjust the temperature of the yak bone protein solution obtained in (1) to 80° C., and use an ultrasonic generator to process ultrasonic waves (frequency 85 kH) for 15 minutes.

[0069] (3) regulating the protein content in the yak bone protein liquid is 8%, adding compound protease according to the percentage by weight of protein weight 0.8% in the yak bone protein liquid and carryin...

Embodiment 2

[0073] Example 2 Preparation method of yak bone protein peptide with ACE inhibitory function

[0074] (1) Choose 500 grams of frozen fresh yak bones, wash them with clean water that meets the drinking water hygiene standards, and use a bone crusher to beat the yak bones into bone particles after cleaning the yak bones, add 1.5 times the total amount of yak bone particles in water Treat at 130°C for 3 hours, obtain yak bone cooking liquid with bone residue removed by centrifugation, cool to 25°C, and remove fat through a liquid separation tank to obtain yak bone protein liquid.

[0075] (2) Adjust the temperature of the yak bone protein liquid obtained in (1) to 82° C., and use an ultrasonic generator to process ultrasonic waves (frequency 90 kH) for 20 minutes.

[0076] (3) regulating the protein content in the yak bone protein liquid is 9%, adding compound protease according to the percentage by weight of protein weight 1.0% in the yak bone protein liquid and carrying out ste...

Embodiment 3

[0080] Example 3 Preparation method of yak bone protein peptide with ACE inhibitory function

[0081] (1) Choose 1000 grams of frozen fresh yak bones, wash them with clean water that meets the drinking water hygiene standards, and use a ox bone grinder to beat the yak bones into bone particles after cleaning the yak bones, add 3.0 times the total amount of yak bone particles in water Treat at 132°C for 3 hours, then centrifuge to obtain yak bone high-temperature cooking liquid without bone slag, cool to 25°C, and remove fat through a liquid separation tank to obtain yak bone protein liquid.

[0082] (2) Adjust the temperature of the yak bone protein solution obtained in (1) to 85° C., and use an ultrasonic generator to process ultrasonic waves (frequency 90 kH) for 25 minutes.

[0083] (3) regulating the protein content in the yak bone protein liquid is 9%, adding compound protease according to the percentage by weight of protein weight 0.6% in the yak bone protein liquid and ...

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Abstract

The invention provides a yak bone protein peptide with an ACE inhibiting function and a preparation method and application thereof. The yak bone protein peptide is prepared from enzymatically hydrolyzed yak bone protein by multiple-step enzymatic hydrolysis by compound protease, and the compound protease consists of alkali protease, papain, trypsin and flavourzyme. The yak bone protein peptide provided by the invention has an excellent ACE inhibiting function, the ACE inhibiting activity (IC50 value) is smaller than 0.32mg / mL, and the yak bone protein peptide can be used as an ACE inhibitor to be applied to special foods and nutritional foods. The preparation method of the yak bone protein peptide provided by the invention is simple, the whole processing course is not added with acid or alkali for regulating the pH value, and the product maintains good functional characteristics and is easier to realize industrialized production.

Description

technical field [0001] The invention relates to the field of animal food processing, more specifically, to a yak bone protein peptide with ACE inhibitory function, its preparation method and application. Background technique [0002] Hypertension is a disease that is extremely harmful to human health and has a long incubation period. In the early stage of hypertension, there are generally no obvious symptoms until clinical signs of heart attack and cerebral blood vessel rupture lead to death. [0003] Angiotensin I converting enzyme (ACE) is a key enzyme of the renin-angiotensin system. Angiotensinogen is converted into angiotensin I under the action of renin, and angiotensin I is converted into angiotensin II under the action of ACE, which stimulates vasoconstriction and causes blood pressure to rise. Inhibition of ACE activity has a positive effect on lowering blood pressure, so the development of effective ACE inhibitors has attracted great attention. Although the ACE ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06A23L33/18A61K38/39A61P9/12
CPCA61K38/39C07K14/78C12P21/06
Inventor 张恒罗永康
Owner ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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