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Polymer microparticle for carrying physiologically active substance and method for preparing same

A technology of physiologically active substances and polymers, which is applied in the field of polymer microparticles loaded with physiologically active substances and its preparation, and can solve the problems of quantitative modification of difficult blocking agents, increased batch-to-batch differences, and cumbersome problems

Active Publication Date: 2017-08-18
MITSUBISHI CHEM MEDIENCE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems such as increased inter-batch variation due to the cumbersome preparation process of immunological analysis reagents, or difficulty in quantifying the amount of modification of the blocking agent.
[0016] Therefore, although the latex particles used for clinical diagnostic drugs seek to improve the stability of the preparation of clinical diagnostic drugs and the accuracy of clinical diagnostic drugs, they have not yet been realized.

Method used

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  • Polymer microparticle for carrying physiologically active substance and method for preparing same
  • Polymer microparticle for carrying physiologically active substance and method for preparing same
  • Polymer microparticle for carrying physiologically active substance and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] "Example 1: Synthesis of emulsifier (amphiphilic block copolymer)"

[0102] In the present embodiment, according to the reaction process formula shown below, the synthetic emulsifier (amphiphilic block polymer: PSt 21 -b-POEGMA 41 -Cl), synthesized by Gabriel primary amine (Gabriel) to synthesize ω-terminally aminated amphiphilic block copolymer PSt 21 -b-POEGMA 41 -NH 2 .

[0103] [chemical formula 4]

[0104]

[0105] Under a nitrogen atmosphere, to 10 g of styrene (Kanto Chemical Co., Inc.) and 1 g of hydroxyethanol bromoisobutyrate (HEBiB) (2-hydroxyethyl-2-bro moisobutyrate) as an initiator, 2 g The 2,2'-bipyridine (bipyridine; bpy) and 0.6g of Cu Br were polymerized at 110°C. In addition, HEBiB synthesized by 2-bromoisobutyryl bromide (2-bromoisobutyryl bromide) and ethylene glycol was used. After the polymerization is complete, use tetrahydrofuran (THF) to dilute and reprecipitate in methanol to obtain PSt 21 -Br.

[0106] 7.0 g of oligo(ethylene glyc...

Embodiment 2

[0110] "Example 2: Synthesis of latex particles using emulsifiers"

[0111] With the PSt of 0.05g obtained in embodiment 1 21 -b-POEGMA 41 -NH 2 It was dissolved in 0.1 g of styrene, and further, 1.2 g of ultrapure water was added. The mixture was heated up to 90° C. and stirred for 10 minutes to obtain a W / O type emulsion.

[0112] Then, stirring was carried out while cooling in an ice bath, and a phase inversion emulsification operation was performed to obtain an O / W type emulsion. 0.03 g of a water-soluble initiator (VA-044: Wako Pure Chemical Industries, Ltd.) was added, and polymerization was performed at 40° C. for 6 hours.

[0113] The particle diameter of the obtained latex particles was measured using a dynamic light scattering device (DLS: ELSZ-1000ZSCK light scattering apparatus tus Otsuka), and it was 52±20 nm.

Embodiment 3

[0114] "Example 3: The influence of the chain length of the hydrophobic segment of the emulsifier on the particle size"

[0115] According to Example 1, the repeating unit (equivalent to n of general formula (1)) having polystyrene 13, 21, 46, 95 on the hydrophobic segment and having 42 in the main chain of the hydrophilic segment , 41, 45, and 36 repeating units (corresponding to m in the general formula (1)) are amphiphilic block polymers shown in Table 1. In addition, OEGMA (polymerization degree: 9) was used for the graft part. By setting the molar ratio (M / I ratio) of the monomer (styrene) / initiator (HEBiB) to the value shown in Table 1, the repeating unit of the hydrophobic segment was controlled. By making the molar ratio of monomer (OEGMA) / initiator (PSt-Br) uniform to 50, control of the repeating unit of the hydrophilic segment was performed.

[0116] Then, according to embodiment 2, synthesize the latex particle of the combination described in table 1, promptly syn...

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Abstract

The invention provides a novel polymer microparticles for carrying a physiologically active substance and a method for preparing the same. The polymer microparticles for carrying a physiologically active substance can provide an analytical reagent which has high analytical precision and sensitivity and can be prepared stably; can easily and precisely control the amount of functional groups carrying the physiologically active substance; can introduce, to latex particle surfaces, a hydrophilic compound for suppressing a nonspecific reaction; and can be prepared to have a narrow and uniform particle size distribution. The polymer microparticles for carrying a physiologically active substance are obtained by polymerizing a monomer, a radical polymerization initiator, and an emulsifier, and the emulsifier is an amphiphilic block polymer expressed by general formula (1) below.

Description

technical field [0001] The invention relates to a polymer microparticle for carrying physiologically active substances and a preparation method thereof. [0002] In addition, "analysis" in this specification includes both of "measurement" to quantitatively or semiquantitatively determine the amount of an analyte, and "detection" to determine the presence or absence of an analyte. Background technique [0003] Today, at the site of clinical diagnostic examination, it is required to measure various substances serving as disease diagnostic indicators in a short time and with high precision for many subjects, and to quickly and accurately feed back the results to the treatment site. For example, analysis that binds physiologically active substances (proteins such as antibodies, enzymes, and acceptors, antigens, nucleic acid substances such as DNA or RNA, or sugar chains, etc.) to the surface of particles, such as immunological detection using antigen-antibody reactions, We carr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/545C08F2/24C08F2/38C08F297/00
CPCC08F2/24C08F293/005C08F2438/01C08F212/08C08F220/306C08F2/38C08J3/12G01N33/545C08F297/00
Inventor 门胁淳谷口龙王佐佐木祐亮小西菜穗
Owner MITSUBISHI CHEM MEDIENCE
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