Peanut rhizosphere bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacillus, applied in the direction of application, bacteria, biocide, etc., to achieve the effect of improving the microbial community structure, increasing the number, increasing the variety and abundance

Active Publication Date: 2017-08-18
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this strain only involves the study of a single antibacterial function, and Bacillus amyloliquefaciens with multiple growth-promoting mechanisms and biological control functions, such as growth-promoting, siderophore production, plant resistance induction, and prevention and control of peanut white silkworm, has not been reported yet. Therefore, it is of great significance to screen strains with multiple growth-promoting mechanisms and biological control functions in peanut production

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  • Peanut rhizosphere bacillus amyloliquefaciens and application thereof
  • Peanut rhizosphere bacillus amyloliquefaciens and application thereof
  • Peanut rhizosphere bacillus amyloliquefaciens and application thereof

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Effect test

Embodiment 1

[0035] Embodiment 1: Isolation, screening of bacterial strain

[0036] 1. Isolation and screening of strains:

[0037] (1) Isolation of strains

[0038] The strain was sieved from the peanut rhizosphere soil, and the soil samples were collected from the peanut planting field in the suburb of Tai'an City, Shandong Province. Carefully shake off the peanut rhizosphere soil into a sterile bag, put it in a low-temperature fresh-keeping box, and process it immediately after bringing it back to the laboratory. Weigh 10 g of the rhizosphere soil sample, put it into 90 mL of sterile water, and shake it for 30 min to make a microbial suspension. Take 1mL of the suspension respectively, and after diluting according to the 10-fold ratio, take 10 -4 、10 -5 and 10 -6 Spread 200 μL of the double dilution on the beef extract peptone plate, let it stand for 10 minutes, and then place it upside down in a constant temperature incubator at 37°C for about 24 hours. Select representative colo...

Embodiment 2

[0056] Embodiment 2: the preparation of bacterial strain Y14 biocontrol agent

[0057] The specific preparation method is as follows:

[0058] Preparation method of Y14 fermentation broth: transfer the Bacillus amyloliquefaciens Y14 preserved on the slant to a test tube containing 10 mL of liquid LB medium, and place it in a constant temperature shaker for 12 hours (temperature: 37° C.; rotation speed: 180 rpm). Insert the activated bacterium liquid into 200mL liquid LB medium according to the inoculation amount of 1% (volume percentage), put it into a shaker and cultivate it for 24h (temperature: 37°C; rotating speed: 180rpm) to obtain the fermentation liquid of the Y14 strain; as When using biocontrol preparations, dilute the fermentation broth with appropriate amount of sterile water to 1×10 8 cfu / ml is enough.

Embodiment 3

[0059] Embodiment 3: Determination of bacterial strain Y14 inhibiting the growth of the pathogenic bacteria of white silkworm

[0060] 1. Table stand-off test

[0061] (1) Materials and methods:

[0062] Plate confrontation test was used to inoculate the activated peanut white silk mycelium block cut by a sterile puncher in the center of a PDA plate, cultured at 28°C for 1 day, and the antagonistic bacteria Y14 was inoculated at a place about 3 cm away from the edge of the white silk mycelium. Only pathogenic bacteria were inoculated as a control, and the antibacterial effect was observed after 3 days of culture. At the same time, the white silk mycelia at the edge of the confrontation were picked, and the growth and morphological changes of the mycelium were observed under a microscope with the white silk mycelium without antagonistic bacteria as a control.

[0063] (2) Test results:

[0064] Through plate confrontation culture, it was found that the strain Y14 had a signi...

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Abstract

The invention discloses bacillus amyloliquefaciens. The bacillus amyloliquefaciens has the classification and name of Bacillus amyloliquefaciens Y14 and is preserved in China General Microbiological Culture Collection Center (CGMCC for short) on November 10th, 2016 with the preservation address of Microbiology Research Institute of China Science Academe, No.3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing and the preservation number of CGMCC NO.13258. The strain has good inhibitory effect on pathogenic bacteria of southern blight and also can induce resistance of a botanical system, iron nutrition is provided for peanuts, a soil microbial community structure also can be improved, and a strong guarantee is provided for production of the peanuts.

Description

technical field [0001] The present invention relates to a strain of Bacillus amyloliquefaciens in peanut rhizosphere and its application, in particular to a strain of Bacillus amyloliquefaciens capable of preventing and treating peanut blight, and simultaneously having the function of promoting growth, improving peanut iron nutrition and rhizosphere microbial community structure, belonging to field of biotechnology. Background technique [0002] The pathogenic bacterium of peanut white silkworm is Selerotium rolfsii Sacc., which spreads by means of sclerotium, mycelium or infected plant debris, causing the roots of plants to rot and infecting a variety of plants. It is a worldwide epidemic soil-borne diseases. Peanut canker sores is a serious disease in my country, and occurs in all major peanut producing areas. Peanut canker sore can cause peanut stem rot and fruit rot, which seriously threatens the yield and quality of peanuts. At present, cultivation measures, breeding ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 丁延芹杜秉海姚良同刘凯汪城墙
Owner SHANDONG AGRICULTURAL UNIVERSITY
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