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Tissue culture seedling method for snake gourds

A technology of trichosanthes and trichosanthes roots, applied in the field of group cultivation of trichosanthes, can solve the problem of poor resistance of trichosanthes, achieve the effects of non-toxicity, accumulation of good traits, and enhancement of resistance

Inactive Publication Date: 2017-08-18
蚌埠清菲农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems mentioned in the background technology, the present invention provides a method for group cultivation of Trichosanthes, which can improve the quality of Trichosanthes, promote high and stable yields, and solve the problem of poor resistance of Trichosanthes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for group seedling cultivation of Trichosanthes quince, comprising the following steps:

[0018] (1) Clean the seed pieces of Trichosanthes quinquefolius root, and germinate them in a sterile environment with a relative air humidity of 70%, a light intensity of 800lx, and a temperature of 25°C, and apply a layer of germination-promoting liquid on the surface of the seed coat. It consists of the following ingredients in parts by weight: 4.5 parts of white sugar, 3.2 parts of egg whites, 1.5 parts of rice milk, 0.2 parts of aloe juice, and 25 parts of soda water;

[0019] (2) Use sterile tweezers to soak the cultivated seed buds in a disinfectant solution for 30 minutes, remove them and rinse them with disinfectant water, peel off the inner basal buds, cut them under a microscope, and keep 0.3mm of the basal bud tip part, put into culture medium;

[0020] (3) During the tissue culture process, the medium components are gradually added with the growth of the seed...

Embodiment 2

[0026] A method for group seedling cultivation of Trichosanthes quince, comprising the following steps:

[0027] (1) Clean the seed blocks of Trichosanthes quinquefolius root, and germinate them in a sterile environment with a relative air humidity of 75%, a light intensity of 900lx, and a temperature of 28°C, and apply a layer of germination-promoting liquid on the surface of the seed coat. It consists of the following ingredients in parts by weight: 4.7 parts of white sugar, 3.3 parts of egg whites, 1.7 parts of rice milk, 0.3 parts of aloe juice, and 27 parts of soda water;

[0028] (2) Use sterile tweezers to soak the cultivated seed buds in a disinfectant solution for 35 minutes, remove them and rinse them with disinfectant water, peel off the inner basal buds, cut them under a microscope, and keep 0.4mm of the apex of the basal buds part, put into culture medium;

[0029] (3) During the tissue culture process, the medium components are gradually added with the growth of...

Embodiment 3

[0035] A method for group seedling cultivation of Trichosanthes quince, comprising the following steps:

[0036] (1) Clean the seed blocks of Trichosanthes quinquefolius root, and germinate them in an aseptic environment with a relative air humidity of 80%, a light intensity of 1000lx, and a temperature of 30°C, and apply a layer of germination-promoting liquid on the surface of the seed coat. It consists of the following ingredients in parts by weight: 5.0 parts of white sugar, 3.5 parts of egg white, 2.0 parts of rice milk, 0.4 part of aloe juice, and 30 parts of soda water;

[0037] (2) Use sterile tweezers to soak the cultivated seed buds in disinfectant solution for 40 minutes, remove them and rinse them with disinfectant water, peel off the inner buds, cut them under a microscope, and keep 0.5 mm of the tip of the buds part, put into culture medium;

[0038] (3) During the tissue culture process, the medium components are gradually added with the growth of the seed buds...

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PUM

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Abstract

The present invention relates to the field of Trichosanthes planting, and discloses a group cultivation method of Trichosanthes trichosanthes, comprising the following steps: (1) selecting Trichosanthes root mass to accelerate germination in a suitable environment. Polysaccharides have the obvious effect of regulating cell division and have strong resistance. The various enzymes and amino acids contained in it make the sprouts strong in rooting, and the survival rate of seedlings is higher than that of seed culture; (2) the germination to The seed buds are peeled off from the basal bud tip, which has the highest hormone level, is non-toxic, has a fast meristem speed, and is easy to cultivate; (3) The ingredients in the medium are added with various nutrients required by the seedlings as the cultivation progresses (4) The seedlings obtained by tissue culture are adapted to the external environment and can be transplanted in the field after stabilization. The method for cultivating seedlings of the group of the present invention improves the character of Trichosanthes trichosanthis, has high and stable yield, and is beneficial to the accumulation of excellent characters.

Description

technical field [0001] The invention relates to the field of Trichosanthes planting, in particular to a group cultivation method of Trichosanthes trichosanthes. Background technique [0002] Gualou, scientific name Trichosanthes, Cucurbitaceae Trichosanthes is a perennial climbing herb, up to 10 meters long. The rhizome is thick, cylindrical, and the outer skin is yellow. Stem much branched, glabrous, leaves alternate, nearly round or heart-shaped, female tendrils axillary, slender, with 2-5 lobes at the top. Leaves alternate, nearly round or heart-shaped, about 7-20 cm in length and width, usually 5-7 palmately lobed or mid-lobed, rarely deeply lobed, or not split but only have coarse teeth of varying sizes, lobes Oblong or oblong-elliptic to ovate-lanceolate, sparsely short-pubescent or glabrous on the surface, acute or short-acuminate at the top, sparsely toothed or notched on the edge. Dioecious; several male flowers are born in racemes on the upper part of the pedunc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 翟明安李香华
Owner 蚌埠清菲农业科技有限公司
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