Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying genetic relationship by developing SNP molecular marker of miscanthus with SLAF-seq technology

A phylogenetic relationship, slaf-seq technology, applied in the field of identification of phylogenetic relationship and phylogenetic evolution research of Miscanthus plants, to achieve the effect of reducing genome complexity, reducing complexity and high accuracy

Inactive Publication Date: 2017-08-11
HUNAN AGRICULTURAL UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, SNP markers are mainly used in some model species (Arabidopsis, rice) or species with reference genomes, and there are still some difficulties and challenges in the research and application of non-model species (Miscanthus)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying genetic relationship by developing SNP molecular marker of miscanthus with SLAF-seq technology
  • Method for identifying genetic relationship by developing SNP molecular marker of miscanthus with SLAF-seq technology
  • Method for identifying genetic relationship by developing SNP molecular marker of miscanthus with SLAF-seq technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] Select 19 offspring samples of Miscanthus whose female parent is known to be Nandi, and samples of Miscanthus, Digi, and Nandi that met the female parent in flowering period were selected as 30 suspected male parents and 1 female parent, a total of 50 materials.

[0029] 1 Reference Genome Determination

[0030] According to the genome size and GC content of Miscanthus plants, the sorghum genome was finally selected as the reference genome for enzyme digestion prediction.

[0031] The specific information is as follows:

[0032] a) Sequencing species information: Miscanthus, the actual genome size is about 2.65Gb, and the GC content is 41.39%;

[0033] b) Reference species information: Sorghum bicolor genome, the assembled genome size is 738.54Mb, GC content is 43.90%, download address: http: / / phytozome.jgi.doe.gov / pz / portal.html#! info? alias = Org_Sbicolor.

[0034] 2 Enzyme digestion scheme determination

[0035] Use enzyme digestion prediction software to perfo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying the genetic relationship by developing an SNP molecular marker of miscanthus with the SLAF-seq technology. The method comprises the following steps: (1) designing a marker development scheme; (2) selecting an optimal digestion scheme; (3) respectively extracting DNA of genomes for digesting, constructing an SLAF-seq sequencing library, and sequencing; (4) developing an SLAF marker for a single sample, then performing similarity clustering on all SLAF markers to obtain an SLAF marker of the cluster; (5) performing allele number, genetic sequence difference and other polymorphic analysis on the cluster SLAF marker, wherein SNP is taken as a polymorphic marker; (6) coding the genotype for parent, representing a character by a genotype, detecting the daughter genotype after the separation type is determined, and representing the SNP coding genotype with a corresponding character; and (7) selecting SNP marker with few sample loss, extracting all SNP sites, calculating the daughter SNP anomalous typing ratio, determining the genetic relationship between daughter and parent according to whether the daughter SNP anomalous typing ratio is higher than the threshold value or not.

Description

technical field [0001] The invention relates to the technical field of plant molecular biology, in particular to a method for identification and systematic evolution research of miscanthus plants. Background technique [0002] Miscanthus Anderss. is a new type of fiber energy plant. Its genome is large, about 2.65Gb, and its sexual reproduction is mainly outcrossing. There are a lot of intraspecific and interspecific hybridization. The genetic background It is quite complicated, which brings great difficulties to phylogenetic research and subsequent gene function development. Molecular marker technology has been widely used in many research fields such as genetics, molecular population genetics, species formation, evolution and kinship, phylogeny and molecular taxonomy. At present, the molecular marker technologies that have been developed include the first-generation molecular marker technologies represented by RFLP, RAPD and AFLP, and the second-generation molecular marke...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 陈智勇胡忠红
Owner HUNAN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com