Multiplex PCR primer sets, kits and applications for detection of Y chromosome microdeletions

Abstract

The invention relates to the field of gene deletion detecting, in particular to a multiplex PCR (polymerase chain reaction) primer set for detection Y chromosome microdeletion, kit and application. The multiplex PCR primer set comprises a universal primer and a specific primer, the universal primer has base sequences shown as SEQ ID NO:1 and SEQ ID NO:2, a 5' terminal of the base sequence shown as the SEQ ID NO:1 is marked with a fluorophore, and the specific primer comprises a primer having base sequences shown as SEQ ID NO:3-SEQ ID NO:22. The defect that conventional multiplex PCR is low in sensitivity and non-specific amplification is overcome; through a method combining a novel stable universal primer-multiplex PCR reaction system with a fluorescent labeling universal primer, an amplification product is subjected to capillary electrophoresis analysis, so that high sensitivity of detection is guaranteed, cost is effectively lowered, and large-sample-size efficient detection can be realized.

Description

technical field [0001] The present invention relates to the field of gene deletion detection, more specifically, to a multiplex PCR primer set for detecting Y chromosome microdeletion, the kit including the multiplex PCR primer set and the multiple PCR primer set and the kit as a method for detecting Y chromosome microdeletion Application of missing reagents. Background technique [0002] According to the research report of the World Health Organization (WHO), about 15% of couples of childbearing age in the world are plagued by infertility (fertility), of which male factors account for about 50%. More than 30% of male infertility is caused by genetic factors, mainly Klinefelter syndrome and Y chromosome microdeletion. Cytogenetic and molecular biology studies have confirmed that microdeletions in the azoospermia factor (AZF) region located on the long arm of the Y chromosome can cause spermatogenesis disorders, leading to azoospermia or severe oligospermia. Vogt et al. div...

AI Technical Summary

Problems solved by technology

The present invention overcomes the shortcomings of traditional multiplex PCR such as low sensitivity and non-specific amplification. Through a novel set of stable universal primer-multiple PCR reaction system combined with fluorescently labeled universal primers, the amplified products are analyzed by capillary electrophoresis to ensure The high sensitivity of the detection effectively reduces the cost, and can realize the efficient detection of large sample volume

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