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Pesticide carbendazim degrading microbial inoculant and preparation method thereof

A technology of microbial bacterial agent and carbendazim, which is applied in the field of pesticide carbendazim-degrading microbial bacterial agent and its preparation, can solve problems such as unreasonable use of chemical pesticides, ecological environment pollution, and excessive pesticide residues, and achieve simple construction and environmental protection. The effect of small impact and low processing cost

Active Publication Date: 2017-08-04
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in the use of chemical pesticides are irrational use of chemical pesticides, serious pesticide residues in agricultural products, high cost of control, serious pollution to the ecological environment, and large-scale pesticide damage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The preparation method of the pesticide carbendazim degrading microbial inoculum of the present invention includes the following steps:

[0021] Step 1: Fermentation:

[0022] Adjust the pH of the liquid fermentation medium A to 6.5-7.0, and perform routine aseptic processing, according to the liquid quality 6-9% aseptic operation to access the Pallidum hominis WNPA2, constant temperature 30 ± 1 ℃, stirring speed 180-200rpm, Ventilation volume 0.27-0.30M 3 / min, tank pressure 0.07-0.08MPa, continuous fermentation for 72-76hr, microscopic examination and counting the number of cells in the fermentation broth up to 1.5-2.0×10 9 Fermentation is terminated when each / mL;

[0023] Step 2: Separation of bacterial cells:

[0024] Collect the Paleococcus hominis WNPA2 fermentation broth of step 1 liquid fermentation medium A, and centrifuge to remove the moisture in the fermentation broth with a yeast separation centrifuge to obtain milky white viscous bacterial cells;

[0025] Step 3: P...

Embodiment 1

[0031] Step 1: Fermentation:

[0032] Adjust the pH of the liquid fermentation medium A to 6.0, and perform conventional aseptic processing, and connect to the Paleococcus hominis WNPA2 according to the aseptic operation of 9% of the liquid quality, the constant temperature is 29℃, the stirring speed is 180rpm, and the ventilation volume is 0.27M 3 / min, tank pressure 0.08MPa, continuous fermentation for 74 hours, microscopic examination and counting of the number of cells in the fermentation broth up to 2.0×10 9 Fermentation is terminated when each / mL;

[0033] The formula of liquid fermentation medium A is as follows:

[0034] Glucose 18g / L, MgSO 4 ·7H 2 O 0.5 g / L, NH 4 NO 3 1.0 g / L, (NH 4 ) 2 SO 4 1.5g / L, NaCl 1.0g / L, K 2 HPO4·3H 2 O 0.5 g / L, MnSO 4 ·2H 2 O 0.4mg / L, the remaining amount of water is fixed to 1L.

[0035] Step 2: Separation of bacterial cells:

[0036] Collect the WNPA2 fermentation broth of Paleococcus hominis in step one liquid fermentation medium A, and centrifuge...

Embodiment 2

[0040] Step 1: Fermentation:

[0041] Adjust the pH of the liquid fermentation medium A to 6.2, and perform routine aseptic processing. According to the aseptic operation of 6% of the liquid quality, it is connected to Paleococcus hominis WNPA2, the constant temperature is 31℃, the stirring speed is 190rpm, and the ventilation volume is 0.28M 3 / min, tank pressure 0.08MPa, continuous fermentation for 76 hours, microscopic examination and counting the number of cells in the fermentation broth up to 1.8×10 9 Fermentation is terminated when each / mL;

[0042] The formula of liquid fermentation medium A is as follows:

[0043] Glucose 15g / L, MgSO 4 ·7H 2 O 0.6 g / L, NH 4 NO 3 1.5 g / L, (NH 4 ) 2 SO 4 2.0 g / L, NaCl1.0g / L, K 2 HPO4·3H 2 O 0.8g / L, MnSO 4 ·2H 2 O 0.5mg / L, the remaining amount of water is fixed to 1L.

[0044] Step 2: Separation of bacterial cells:

[0045] Collect the Paleococcus hominis WNPA2 fermentation broth of step 1 liquid fermentation medium A, and centrifuge to remove th...

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Abstract

The invention relates to a pesticide carbendazim degrading microbial inoculant and a preparation method thereof. In an agricultural production process, as pesticides are excessively used, high residues of pesticides in agricultural products and agricultural soil are caused, which brings a certain problem to safety of the agricultural products and sustainable development of the agricultural soil. A human ochrobactrum WNPA2 is cultured in a special liquid fermenting culture medium, fermentation liquor is collected for centrifugal separation to remove moisture in the fermenting liquor to obtain milk white thick somatic cells; by adding glycerinum and diatomite which are uniformly stirred and mixed, a white solid preparation, namely, the target product, is obtained. The preparation method provided by the invention is an efficient and economical environment-friendly pesticide residue treatment technology. The carbendazim degrading rate can reach 50% above, and the pesticide carbendazim degrading microbial inoculant has the advantages of being low in treatment cost, simple to construct, free of secondary pollution, small in environmental influence, etc.

Description

Technical field [0001] The invention belongs to the technical field of soil bioremediation, and specifically relates to a pesticide carbendazim degradation microbial inoculum and a preparation method thereof. Background technique [0002] As an important means to ensure a bumper harvest in agriculture, chemical pesticides play an important role in agricultural production. The main problems in the use of chemical pesticides are the unreasonable use of chemical pesticides, the serious excess of pesticide residues in agricultural products, and the high cost of prevention and control, which have caused serious pollution to the ecological environment and caused large-scale phytotoxicity. Microbial remediation is a biological measure that uses the metabolic absorption, transformation, removal or degradation of chemical pesticide components of specific microorganisms to achieve environmental purification and restoration of ecological effects. It has the characteristics of high efficien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C12N1/20C09K101/00C12R1/01
Inventor 陈锐孙晓宇沈卫荣路鹏鹏赵玲侠瞿佳
Owner MICROBIOLOGY INST OF SHAANXI
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